Vineetha M S, Bhavya J, Veena S M, Mirajkar Kiran K, Muddapur Uday, Ananthraju K S, Zameer Farhan, More Sunil S
School of Basic and Applied Sciences, Dayananda Sagar University, K.S Layout, Bangalore 560 111, Karnataka, India.
Department of Biotechnology, Sapthagiri College of Engineering, Bangalore 560 057, Karnataka, India.
Saudi Pharm J. 2020 Jun;28(6):692-697. doi: 10.1016/j.jsps.2020.04.010. Epub 2020 Apr 25.
root is traditionally used and practiced among few Indian tribes as an antidote for snakebites.
To combat and neutralize venom using methanolic root extract of and to explore its efficacy on venom biomarkers in search of newer herbal antidote or first-aid-point of care for therapeutics.Materialization.Pharmacological activities such as fibrinogenolytic, direct and indirect hemolytic activities for the neutralization of the venom were evaluated. Lethal toxicity annulation studies were performed using the murine model by pre-incubation and post-treatment protocols. Further, the neutralization of edema and myotoxicity were also evaluated.
Electrophoretic analysis revealed that the complete neutralization of fibrinogen degradation was observed at 1:10 (w/w) (venom to extract). exhibited an effective dose (ED) value of 87.20 µg/mL for venom-induced hemolysis. Venom at 2 µg concentration produced 11 mm of hemolytic radiance and was neutralized at 1:20 (w/w) venom to extract concentration. The survival time and the neurotoxic symptoms in mice were concluded to be delayed by both the methods of lethal toxicity inhibition using methanol extract. The edema ratio reduced the venom to extract ratio of 1:20 (w/w) from 173 ± 45% to 133.61% when subjected to 5 µg of venom concentration. The plant extract significantly neutralized the myotoxic activity.
methanolic root extract could be a potent contributor in the effective treatment of venom-induced toxicity.
在少数印度部落中，传统上使用[植物名称]的根来治疗蛇咬伤。
使用[植物名称]的甲醇根提取物对抗和中和毒液，并探索其对毒液生物标志物的功效，以寻找新的草药解毒剂或治疗的即时护理急救方法。实现方式。评估了诸如纤维蛋白溶解、直接和间接溶血活性等药理学活性以中和毒液。通过预孵育和后处理方案，使用小鼠模型进行了致死毒性消除研究。此外，还评估了水肿和肌毒性的中和情况。
电泳分析显示，在1:10（w/w）（毒液与提取物）时观察到纤维蛋白原降解的完全中和。[植物名称]对毒液诱导的溶血表现出87.20μg/mL的有效剂量（ED）值。2μg浓度的毒液产生11mm的溶血半径，在1:20（w/w）的毒液与提取物浓度下被中和。使用甲醇提取物的两种致死毒性抑制方法均使小鼠的存活时间和神经毒性症状延迟。当毒液浓度为5μg时，水肿率将毒液与提取物的比例从173±45%降低到133.61%。植物提取物显著中和了肌毒性活性。
[植物名称]的甲醇根提取物可能是有效治疗[植物名称]毒液诱导毒性的有力贡献者。
