Lin Shudai, Fang Lingzhao, Kang Xiaolong, Liu Shuli, Liu Mei, Connor Erin E, Baldwin Ransom L, Liu George, Li Cong-Jun
Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science of South China Agricultural University, Guangzhou, 510642, China.
Heliyon. 2020 Jun 9;6(6):e04112. doi: 10.1016/j.heliyon.2020.e04112. eCollection 2020 Jun.
As a critical and high-value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue. The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression were assessed. Correlated gene networks elucidated the putative roles and mechanisms of butyrate action in rumen epithelial development. The top networks perturbed by butyrate were associated with epithelial tissue development. Additionally, two critical upstream regulators, E2F1 and TGFB1, were identified to play critical roles in the differentiation, development, and growth of epithelial cells. Significant expression changes of upstream regulators and transcription factors provided further evidence in support that butyrate plays a specific and central role in regulating genomic and epigenomic activities influencing rumen development. This work is the essential component to obtain a complete global landscape of regulatory elements in cattle and to explore the dynamics of chromatin states in rumen epithelial cells induced by butyrate at early developmental stages.
作为研究瘤胃发育的关键且高价值工具，我们从两周龄的荷斯坦公牛犊瘤胃上皮组织建立了稳定的瘤胃上皮原代细胞（REPC）培养体系。评估了REPC的转录组图谱以及丁酸盐对基因表达的直接影响。相关基因网络阐明了丁酸盐在瘤胃上皮发育中的假定作用和机制。受丁酸盐干扰的顶级网络与上皮组织发育相关。此外，还确定了两个关键的上游调节因子E2F1和TGFB1在上皮细胞的分化、发育和生长中起关键作用。上游调节因子和转录因子的显著表达变化进一步证明丁酸盐在调节影响瘤胃发育的基因组和表观基因组活动中发挥着特定且核心的作用。这项工作是获得牛调控元件完整全局图谱以及探索早期发育阶段丁酸盐诱导的瘤胃上皮细胞染色质状态动态变化的重要组成部分。
