Baldwin Ransom L, Wu Sitao, Li Weizhong, Li Congjun, Bequette Brian J, Li Robert W
USDA-ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA.
Gene Regul Syst Bio. 2012;6:67-80. doi: 10.4137/GRSB.S9687. Epub 2012 May 16.
Short-chain fatty acids (SCFAs), such as butyrate, produced by gut microorganisms, play a critical role in energy metabolism and physiology of ruminants as well as in human health. In this study, the temporal effect of elevated butyrate concentrations on the transcriptome of the rumen epithelium was quantified via serial biopsy sampling using RNA-seq technology. The mean number of genes transcribed in the rumen epithelial transcriptome was 17,323.63 ± 277.20 (±SD; N = 24) while the core transcriptome consisted of 15,025 genes. Collectively, 80 genes were identified as being significantly impacted by butyrate infusion across all time points sampled. Maximal transcriptional effect of butyrate on the rumen epithelium was observed at the 72-h infusion when the abundance of 58 genes was altered. The initial reaction of the rumen epithelium to elevated exogenous butyrate may represent a stress response as Gene Ontology (GO) terms identified were predominantly related to responses to bacteria and biotic stimuli. An algorithm for the reconstruction of accurate cellular networks (ARACNE) inferred regulatory gene networks with 113,738 direct interactions in the butyrate-epithelium interactome using a combined cutoff of an error tolerance (ɛ = 0.10) and a stringent P-value threshold of mutual information (5.0 × 10(-11)). Several regulatory networks were controlled by transcription factors, such as CREBBP and TTF2, which were regulated by butyrate. Our findings provide insight into the regulation of butyrate transport and metabolism in the rumen epithelium, which will guide our future efforts in exploiting potential beneficial effect of butyrate in animal well-being and human health.
肠道微生物产生的短链脂肪酸（SCFAs），如丁酸，在反刍动物的能量代谢和生理机能以及人类健康中发挥着关键作用。在本研究中，利用RNA测序技术通过连续活检采样对瘤胃上皮转录组中丁酸浓度升高的时间效应进行了定量分析。瘤胃上皮转录组中平均转录基因数为17323.63±277.20（±标准差；N = 24），而核心转录组由15025个基因组成。总体而言，在所有采样时间点，共鉴定出80个受丁酸盐注入显著影响的基因。在注入72小时时观察到丁酸对瘤胃上皮的最大转录效应，此时58个基因的丰度发生了改变。瘤胃上皮对外源丁酸升高的初始反应可能代表一种应激反应，因为所鉴定的基因本体（GO）术语主要与对细菌和生物刺激的反应有关。一种用于重建精确细胞网络的算法（ARACNE），使用误差容限（ɛ = 0.10）和严格的互信息P值阈值（5.0×10⁻¹¹）的组合截止值，推断出丁酸-上皮细胞相互作用组中有113738个直接相互作用的调控基因网络。几个调控网络由转录因子控制，如CREBBP和TTF2，它们受丁酸调控。我们的研究结果为瘤胃上皮中丁酸转运和代谢的调控提供了见解，这将指导我们未来努力开发丁酸在动物健康和人类健康方面的潜在有益作用。
