LncRNA AWPPH 在骨质疏松症中表达下调，并调节 I 型胶原α1 和α2 的比例。
LncRNA AWPPH is downregulated in osteoporosis and regulates type I collagen α1 and α2 ratio.
机构信息
Department of Orthopedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, P. R. China.
出版信息
Arch Physiol Biochem. 2022 Oct;128(5):1297-1301. doi: 10.1080/13813455.2020.1767150. Epub 2020 Jun 17.
Normal ratio of type I collagen α1 to α2 (2:1) maintains normal bone microarchitecture. Altered ratios lead to formation of collagen homotrimers and deteriorated bone microarchitecture. In this study, we aimed to investigate the role of lncRNA AWPPH in osteoporosis. We observed that the expression of lncRNA AWPPH was downregulated in osteoporosis patients than that in healthy controls. Downregulated expression of lncRNA AWPPH distinguished osteoporosis patients from healthy controls. cell experiments showed that knockdown of lncRNA AWPPH led to upregulated α1 but downregulated expression of α2 in osteoblasts, which made the α1 to α2 ratio higher than 2:1. In contrast, overexpression of lncRNA AWPPH led to downregulated α1 but upregulated α2 in osteoblasts, which made the α1 to α2 ratio lower than 2:1. Therefore, lncRNA AWPPH is downregulated in osteoporosis and altered the expression of lncRNA AWPPH regulates type I collagen α1 and α2 ratio in osteoblasts.
正常的 I 型胶原α1 与α2 的比例（2:1）维持正常的骨微观结构。比值的改变导致胶原同源三聚体的形成和骨微观结构的恶化。在这项研究中，我们旨在研究 lncRNA AWPPH 在骨质疏松症中的作用。我们观察到，与健康对照组相比，骨质疏松症患者的 lncRNA AWPPH 表达下调。lncRNA AWPPH 的下调表达将骨质疏松症患者与健康对照组区分开来。细胞实验表明，lncRNA AWPPH 的敲低导致成骨细胞中α1 的上调和α2 的下调，使α1 与α2 的比值高于 2:1。相反，lncRNA AWPPH 的过表达导致成骨细胞中α1 的下调和α2 的上调，使α1 与α2 的比值低于 2:1。因此，lncRNA AWPPH 在骨质疏松症中下调，改变 lncRNA AWPPH 的表达调节成骨细胞中 I 型胶原α1 和α2 的比值。
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