Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, Shandong, China.
Department of Radiotherapy, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, Shandong, China.
J Cell Biochem. 2019 Sep;120(9):14860-14866. doi: 10.1002/jcb.28747. Epub 2019 Apr 29.
Long-non-coding RNAs (lncRNA) AWPPH promotes the progression of liver and bladder cancer, indicating its oncogenic role. The current study aimed to explore the involvement of AWPPH in triple-negative breast cancer (TNBC). In the current study, we found that plasma levels of lncRNA AWPPH and microRNA-21 (miRNA-21) were upregulated in patients with TNBC than in healthy controls, and the upregulation of plasma lncRNA AWPPH and miRNA-21 distinguished early-stage patients with TNBC from healthy controls. Plasma levels of lncRNA AWPPH and miRNA-21 were significantly and positively correlated in both patients with TNBC and healthy controls. LncRNA AWPPH and miRNA-21 overexpression led to promoted cancer cells proliferation and improved cancer cell viability under carboplatin treatment, while lncRNA AWPPH small interfering RNA (siRNA) silencing played an opposite role. In addition, miRNA-21 overexpression attenuated the effects of lncRNA AWPPH siRNA silencing on of cancer cell behaviors. LncRNA AWPPH overexpression led to upregulated miRNA-21 in TNBC cells, while miRNA-21 overexpression also led to significantly upregulated lncRNA AWPPH expression. Therefore, lncRNA AWPPH and miRNA-21 may regulate cancer cell proliferation and chemosensitivity in TNBC by interacting with each other.
长链非编码 RNA（lncRNA）AWPPH 促进肝癌和膀胱癌的进展，表明其具有致癌作用。本研究旨在探讨 AWPPH 在三阴性乳腺癌（TNBC）中的作用。本研究发现，与健康对照组相比，TNBC 患者的血浆 lncRNA AWPPH 和 microRNA-21（miRNA-21）水平上调，且上调的血浆 lncRNA AWPPH 和 miRNA-21 可将早期 TNBC 患者与健康对照组区分开。lncRNA AWPPH 和 miRNA-21 在 TNBC 患者和健康对照组中的表达水平呈显著正相关。lncRNA AWPPH 和 miRNA-21 的过表达导致在顺铂治疗下癌细胞增殖增强和活力提高，而 lncRNA AWPPH 小干扰 RNA（siRNA）沉默则起到相反的作用。此外，miRNA-21 的过表达减弱了 lncRNA AWPPH siRNA 沉默对癌细胞行为的影响。lncRNA AWPPH 的过表达导致 TNBC 细胞中 miRNA-21 的上调，而 miRNA-21 的过表达也导致 lncRNA AWPPH 表达的显著上调。因此，lncRNA AWPPH 和 miRNA-21 可能通过相互作用调节 TNBC 中的癌细胞增殖和化疗敏感性。
