Department of Oncology, Dalian Medical University, Dalian City, Liaoning Province 116044, P.R. China.
Department of Breast oncology, The Second Hospital of Dalian Medical University, Dalian City, Liaoning Province 116027, P.R. China.
Biosci Rep. 2018 Nov 28;38(6). doi: 10.1042/BSR20181223. Print 2018 Dec 21.
LncRNA AWPPH is a recently identified critical player in the development of several types of human malignancies, our study aimed to investigate the role of AWPPH in triple-negative breast cancer. In the present study, expression of AWPPH in tumor tissues and adjacent healthy tissues of patients with triple-negative breast cancer as well as in plasma of both patients and healthy people was detected by qRT-PCR. Application potentials of AWPPH in the diagnosis and prognosis of triple-negative breast cancer were evaluated by ROC curve analysis and survival curve analysis, respectively. AWPPH expression vectors and frizzled homolog 7 (FZD7) siRNAs were transfected into cells of human breast cancer cell lines. Expression of FZD7 was detected by Western blot, and cell proliferation was detected using CCK-8 kit. We observed that AWPPH was significantly up-regulated in tumor tissues than in paired adjacent healthy tissues of patients. Plasma levels of AWPPH were higher in patients than in controls. AWPPH overexpression promoted cancer cell proliferation and up-regulated FZD7 expression. FZD7 siRNA silencing inhibited cancer cell proliferation but did not significantly affect AWPPH expression. Compared with cells with AWPPH overexpression alone, cells with both FZD7 siRNA silencing and AWPPH overexpression showed significantly reduced proliferation ability. We conclude that LncRNA AWPPH may promote the growth of triple-negative breast cancer by up-regulating FZD7.
LncRNA AWPPH 是最近被鉴定出的在多种人类恶性肿瘤发展中起关键作用的因子，本研究旨在研究 AWPPH 在三阴性乳腺癌中的作用。 在本研究中，通过 qRT-PCR 检测了三阴性乳腺癌患者肿瘤组织和相邻正常组织以及患者和健康人血浆中 AWPPH 的表达。通过 ROC 曲线分析和生存曲线分析分别评估了 AWPPH 在三阴性乳腺癌诊断和预后中的应用潜力。将 AWPPH 表达载体和卷曲蛋白同源物 7 (FZD7) siRNA 转染入人乳腺癌细胞系的细胞中。通过 Western blot 检测 FZD7 的表达，通过 CCK-8 试剂盒检测细胞增殖。 我们观察到 AWPPH 在肿瘤组织中的表达明显高于患者配对的相邻正常组织。患者血浆中的 AWPPH 水平高于对照组。AWPPH 过表达促进癌细胞增殖并上调 FZD7 表达。FZD7 siRNA 沉默抑制癌细胞增殖，但对 AWPPH 表达没有显著影响。与单独过表达 AWPPH 的细胞相比，同时沉默 FZD7 和过表达 AWPPH 的细胞增殖能力明显降低。 我们得出结论，LncRNA AWPPH 可能通过上调 FZD7 促进三阴性乳腺癌的生长。
