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合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过磷酸三酯中间体制备m-6-2-A-C-C-U-C-C和A-C-C-U-C-m-4-2C

Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of m-6-2-A-C-C-U-C-C and A-C-C-U-C-m-4-2C via phosphotriester intermediates.

作者信息

van Boom J H, Burgers P M, van der Marel G, Wille G

出版信息

Nucleic Acids Res. 1977 Mar;4(3):747-59. doi: 10.1093/nar/4.3.747.

DOI:10.1093/nar/4.3.747
PMID:325521
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342477/
Abstract

The synthesis of two fully-protected hexanucleotides (11a and 11b) via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 5'-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3'leads to5' internucleotide linkages.

摘要

报道了通过磷酸三酯法合成两种完全保护的六核苷酸（11a和11b），该方法基于对核苷酸间连接使用两种保护基团，即5'-末端的一个2,2,2-三溴乙基和其余连接的四个2-氯苯基。通过逐步两步磷酸三酯法组装的六核苷酸11a和11b可以方便地脱保护，得到仅含有3'到5'核苷酸间连接的两种六聚体12a和12b。

相似文献

1
Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of m-6-2-A-C-C-U-C-C and A-C-C-U-C-m-4-2C via phosphotriester intermediates.合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过磷酸三酯中间体制备m-6-2-A-C-C-U-C-C和A-C-C-U-C-m-4-2C
Nucleic Acids Res. 1977 Mar;4(3):747-59. doi: 10.1093/nar/4.3.747.
2
Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过改良的磷酸三酯法制备A-C-C-U-C-C。
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引用本文的文献

1
The chemical synthesis of the anticodon loop of an eukaryotic initiator tRNA containing the hypermodified nucleoside N6-/N-threonylcarbonyl/-adenosine/t6A/1.包含超修饰核苷N6-（N-苏氨甲酰）-腺苷（t6A）的真核起始tRNA反密码子环的化学合成。
Nucleic Acids Res. 1978 Jun;5(6):1889-905. doi: 10.1093/nar/5.6.1889.

本文引用的文献

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The synthesis of oligoribonucleotides. II. Methoxymethylidene derivatives of ribonucleosides and 5'-ribonucleotides.寡核糖核苷酸的合成。II. 核糖核苷和5'-核糖核苷酸的甲氧基亚甲基衍生物。
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Deoxy oligonucleotide synthesis via the triester method.通过三酯法合成脱氧寡核苷酸。
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The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.大肠杆菌16S核糖体RNA的3'末端序列：与无义三联体及核糖体结合位点的互补性
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Inactivation of bacterial ribosomes in vivo and in vitro by cloacin DF13.克洛辛DF13在体内和体外对细菌核糖体的灭活作用。
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Mechanism of kasugamycin resistance in Escherichia coli.大肠杆菌中春雷霉素抗性的机制。
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Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells.体外大肠杆菌素E3对核糖体的特异性失活作用及产大肠杆菌素细胞中的免疫机制
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