Adamiak R W, Biala E, Kierzek R, Kraszewski A, Markiewicz W T, Stawiński J
Nucleic Acids Res. 1977 Jul;4(7):2321-9. doi: 10.1093/nar/4.7.2321.
Zinc/acetylacetone/pyridine treatment has been designed as a very efficient method for removal of 2,2,2,-trichloroethyl group from phosphoesters. Internucleotide and terminal 2,2,2-trichloroethylphosphotriesters were transformed to corresponding diesters quantitatively. Much less reactive 2,2,2-trichloroethylphosphodiesters produced monoesters with ca. 90% yield. 31P NMR spectroscopy has been proposed as a new tool for analysis of removal of internucleotide phosphate protecting groups-a crucial step in oligonucleotides synthesis via phosphotriester approach.
锌/乙酰丙酮/吡啶处理方法被设计为一种从磷酸酯中去除2,2,2-三氯乙基基团的高效方法。核苷酸间和末端的2,2,2-三氯乙基磷酸三酯被定量转化为相应的二酯。活性较低的2,2,2-三氯乙基磷酸二酯生成单酯的产率约为90%。31P核磁共振光谱已被提议作为分析核苷酸间磷酸保护基团去除的一种新工具,这是通过磷酸三酯法合成寡核苷酸的关键步骤。