Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, China.
Pharmacology Department, Sanquan College of Xinxiang Medical University, Xinxiang, 453000, China.
BMC Complement Med Ther. 2020 Jun 17;20(1):187. doi: 10.1186/s12906-020-2833-9.
To evaluate the in vitro antibacterial effect of flavonoids-rich Ziziphus jujuba Mill. extract (FZM) against the formation of bacterial biofilms (BBFs) in Staphylococcus aureus.
FZM can effectively inhibit the formation of S. aureus biofilms in vitro. Morphological observation showed a decrease in both biofilm adhesion and thickness. Results of confocal laser scanning microscopy used to detect the thickness of the BBFs showed that FZM treatment reduced the thickness of the BBFs. Furthermore, after the Image-Pro Plus v.6.0 analysis of the fluorescence intensity, FZM treatment reduced the thickness of the BBFs as well as the proportion of green fluorescence. Scanning electron microscopy showed that FZM can disrupt the channels available for substance exchange in the biofilm, thus exposing the bacterial cells and damaging its three-dimensional structures.
FZM can inhibit biofilm formation, improve the bacterial pH environment, and eliminate the hydrophobic effect of reactive oxygen species and flavonoids.
评估富含黄酮的酸枣提取物（FZM）对金黄色葡萄球菌生物膜（BBF）形成的体外抗菌作用。
FZM 能有效抑制金黄色葡萄球菌生物膜的体外形成。形态学观察显示，生物膜的黏附和厚度均降低。共聚焦激光扫描显微镜检测 BBF 厚度的结果表明，FZM 处理减少了 BBF 的厚度。此外，通过 Image-Pro Plus v.6.0 分析荧光强度，FZM 处理减少了 BBF 的厚度以及绿色荧光的比例。扫描电子显微镜显示，FZM 可以破坏生物膜中物质交换的通道，从而暴露细菌细胞并破坏其三维结构。
FZM 可以抑制生物膜的形成，改善细菌的 pH 环境，并消除活性氧和黄酮的疏水效应。
