Department of Physics, State University of Maringá, Maringá, Paraná, Brazil.
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Avenida Colombo, 5.790, Bloco H67, Sala 11, Jardim Universitário, Maringá, Paraná, 87020-900, Brazil.
Radiat Oncol. 2020 Jun 17;15(1):154. doi: 10.1186/s13014-020-01598-0.
Healthcare workers occupationally exposed to F-FDG cannot wear protective equipment, such as lead aprons, since the interaction between high energy radiation (511 keV) and metal increases the dose of radiation absorption. The objective of this study was to evaluate the shielding efficacy of a plastic polymer against the toxicogenomic effects of ionizing radiation in human lymphocytes, using cytokinesis-block micronucleus assays.
Human peripheral blood lymphocytes were isolated from three subjects and cultured under standard conditions. The cultures were exposed to 300 mCi of F-FDG at a distance of 10 cm for 10 min, in the absence of shielding or with lead, polymer, and lead + polymer shields.
Lead shielding was found to increase the number of counts detected by Geiger-Müller radiation monitors as a consequence of the photoelectron effect. Conversely, the lead + polymer shield reduced the number of counts. The lead, polymer, and lead + polymer shields significantly reduced the frequency of micronuclei, nucleoplasmic bridges, and nuclear buds induced by ionizing radiation. Regarding cytotoxicity, only the lead + polymer shield re-established the cell cycle at the level observed for the negative control.
Lead aprons that are internally coated with polymer increased the radiological protection of individuals occupationally exposed to F-FDG PET/CT, especially during examinations.
职业性接触 F-FDG 的医护人员不能穿戴防护设备，如铅围裙，因为高能射线（511keV）与金属的相互作用会增加辐射吸收剂量。本研究的目的是使用细胞遗传学 - 微核检测，评估塑料聚合物对人淋巴细胞电离辐射致毒基因效应的屏蔽效果。
从 3 名研究对象中分离外周血淋巴细胞，并在标准条件下培养。在没有屏蔽的情况下或使用铅、聚合物和铅 + 聚合物屏蔽的情况下，培养物在距离 10cm 处暴露于 300mCi 的 F-FDG 下 10 分钟。
铅屏蔽会因光电效应而增加盖革 - 米勒辐射监测器检测到的计数。相反，铅 + 聚合物屏蔽会减少计数。铅、聚合物和铅 + 聚合物屏蔽可显著降低电离辐射诱导的微核、核质桥和核芽的频率。关于细胞毒性，只有铅 + 聚合物屏蔽才能使细胞周期恢复到阴性对照的水平。
内部涂有聚合物的铅围裙增加了职业性接触 F-FDG PET/CT 的个体的放射防护，特别是在检查期间。
