[急性髓系白血病患者T细胞上PD-1、TIM-3和VISTA的表达及意义]

[Expression and Significance of PD-1, TIM-3 and VISTA on T Cell of Acute Myeloid Leukemia Patients].

作者信息

Ge Meng-Jun, Xu Kai-Lin, Xu Ting, Tang Ya-Nan, Li ZHen-Yu, Yan Zhi-Ling, Sun Hai-Ying, Cheng Hai, Zhu Feng, Sang Wei, Huang Yi-Hong, Qiu Ting-Ting, Li De-Peng

机构信息

Department of Hematology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China.

Department of Hematology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, Jiangsu Province, China,E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Jun;28(3):748-752. doi: 10.19746/j.cnki.issn.1009-2137.2020.03.006.

DOI:10.19746/j.cnki.issn.1009-2137.2020.03.006

PMID:32552931

Abstract

OBJECTIVE

To study the expression of multiple negative costimulatory molecules on peripheral blood T cells in patients with acute myeloid leukemia (AML) and its affection on prognosis.

METHODS

The peripheral blood samples from patients with newly diagnosed AML, complete remission (CR), and no-remission (NR) were collected, the expression levels PD-1、VISTA and TIM-3 in CD4 and CD8 T cells were detected by flow cytometry , and the clinical data of patients were analyzed.

RESULTS

The expression levels of PD-1、VISTA and TIM-3 of CD4 and CD8 T cells in the newly diagnosed AML patients were significantly higher than those in control group (P＜0.05). The expression levels of PD-1、TIM-3 and VISTA of CD4 and CD8 T cells in the CR group were significantly lower than those in newly diagnosed and the NR group (P＜0.05). The TIM-3 expression level positively correlated with VISTA expression level of CD4 and CD8 T cells in newly diagnosed AML patients (r=0.85 and 0.73). The VISTA and PD-1 expression level of CD4 T cells in newly diagnosed AML, NR after first induction chemotherapy and high risk patients significantly increased (P＜0.05), the TIM-3 expression level of CD8 T cells in high risk group significantly increased (P＜0.05), and the VISTA expression level of CD8 T cells in CBFβ-MYH11 mutation-positive group significantly decreased (P＜0.05).

CONCLUSION

The expression of PD-1、TIM-3 and VISTA in AML peripheral blood T cells may be involved in the immune escape of AML and can be the targets of treatment for acute myeloid leukemia patients.

摘要

目的

研究急性髓系白血病（AML）患者外周血T细胞上多种负性共刺激分子的表达及其对预后的影响。

方法

收集新诊断AML、完全缓解（CR）及未缓解（NR）患者的外周血样本，采用流式细胞术检测CD4和CD8 T细胞中PD-1、VISTA和TIM-3的表达水平，并分析患者的临床资料。

结果

新诊断AML患者CD4和CD8 T细胞中PD-1、VISTA和TIM-3的表达水平显著高于对照组（P＜0.05）。CR组CD4和CD8 T细胞中PD-1、TIM-3和VISTA的表达水平显著低于新诊断组和NR组（P＜0.05）。新诊断AML患者CD4和CD8 T细胞中TIM-3表达水平与VISTA表达水平呈正相关（r=0.85和0.73）。新诊断AML、首次诱导化疗后未缓解及高危患者CD4 T细胞中VISTA和PD-1表达水平显著升高（P＜0.05），高危组CD8 T细胞中TIM-3表达水平显著升高（P＜0.05），CBFβ-MYH11突变阳性组CD8 T细胞中VISTA表达水平显著降低（P＜0.05）。