Vasylkivska Maryna, Patakova Petra
Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28, Prague, Czech Republic.
Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28, Prague, Czech Republic.
J Biotechnol. 2020 Aug 20;320:17-27. doi: 10.1016/j.jbiotec.2020.06.008. Epub 2020 Jun 15.
N-butanol, a valued solvent and potential fuel extender, could possibly be produced by fermentation using either native producers, i.e. solventogenic Clostridia, or engineered platform organisms such as Escherichia coli or Pseudomonas species, if the main process obstacle, a low final butanol concentration, could be overcome. A low final concentration of butanol is the result of its high toxicity to production cells. Nevertheless, bacteria have developed several mechanisms to cope with this toxicity and one of them is active butanol efflux. This review presents information about a few well characterized butanol efflux pumps from Gram-negative bacteria (P. putida and E. coli) and summarizes knowledge about putative butanol efflux systems in Gram-positive bacteria.
正丁醇是一种有价值的溶剂和潜在的燃料添加剂，如果能够克服主要工艺障碍——最终丁醇浓度较低，那么它有可能通过使用天然生产者（即产溶剂梭菌）或工程化平台生物（如大肠杆菌或假单胞菌属）进行发酵来生产。最终丁醇浓度较低是由于其对生产细胞具有高毒性。然而，细菌已经发展出几种应对这种毒性的机制，其中之一是活性丁醇外排。本文综述了关于革兰氏阴性菌（恶臭假单胞菌和大肠杆菌）中一些特征明确的丁醇外排泵的信息，并总结了革兰氏阳性菌中假定的丁醇外排系统的相关知识。
