Yang S F, Niu M C
Proc Natl Acad Sci U S A. 1977 May;74(5):1894-8. doi: 10.1073/pnas.74.5.1894.
Messenger RNA was isolated, by means of its attachment to poly(A), from calf liver and the livers of mice, rats, and chickens. When injected into the uterine lumen of immature or spayed mice, both mouse albumin and the albumins characteristic of the species donating the mRNA were synthesized. Studies with inhibitors disclosed that puromycin blocked the synthesis of both albumin species, while dactinomycin affected only mouse albumin synthesis. It appears, therefore, that in the experimental situation used, the function of exogenous liver mRNA is 2-fold: (i) it programs the synthesis of alien albumin in uterine epithelial cells, and (ii) it stimulates the synthesis of mRNA in epithelial cells. The mRNA thus produced primes the synthesis of mouse albumin.
信使核糖核酸（Messenger RNA）通过其与多聚腺苷酸（poly(A)）的结合，从小牛肝脏以及小鼠、大鼠和鸡的肝脏中分离出来。当将其注入未成熟或已切除卵巢的小鼠子宫腔时，小鼠白蛋白以及提供信使核糖核酸的物种所特有的白蛋白均会合成。用抑制剂进行的研究表明，嘌呤霉素会阻断这两种白蛋白的合成，而放线菌素仅影响小鼠白蛋白的合成。因此，在所用的实验情况下，外源性肝脏信使核糖核酸的功能似乎有两个方面：（i）它指导子宫上皮细胞中异种白蛋白的合成，（ii）它刺激上皮细胞中信使核糖核酸的合成。由此产生的信使核糖核酸引发小鼠白蛋白的合成。
