大鼠肝脏多核糖体的生理学。稳定多核糖体的蛋白质合成。
Physiology of rat-liver polysomes. Protein synthesis by stable polysomes.
作者信息
Wilson S H, Hill H Z, Hoagland M B
出版信息
Biochem J. 1967 May;103(2):567-72. doi: 10.1042/bj1030567.
Certain qualitative aspects of protein synthesis in the livers of starved, starved-re-fed and actinomycin D-treated rats have been examined by polyacrylamide-gel electrophoresis. Animals were exposed to a mixture of (14)C-labelled acids for 18-20min. and killed, and an ultrasonic extract of newly formed protein in microsomal vesicles was prepared and examined by gel electrophoresis. In normal and starved-re-fed animals, 27% of the newly synthesized protein was albumin. During starvation, when RNA synthesis was decreased, the percentage of newly formed protein as albumin rose. After actinomycin D treatment of starved-re-fed rats, when only stable messenger RNA persisted in the cytoplasm, albumin synthesis increased to 63% of the total. This finding suggested that albumin was the primary protein synthesized on stable messenger RNA.
通过聚丙烯酰胺凝胶电泳对饥饿、饥饿再喂食以及用放线菌素D处理的大鼠肝脏中蛋白质合成的某些定性方面进行了研究。将动物暴露于含（14）C标记酸的混合物中18 - 20分钟，然后处死，制备微粒体囊泡中新合成蛋白质的超声提取物，并通过凝胶电泳进行检测。在正常和饥饿再喂食的动物中，新合成蛋白质的27%是白蛋白。在饥饿期间，当RNA合成减少时，新形成的蛋白质中白蛋白的百分比上升。在用放线菌素D处理饥饿再喂食的大鼠后，当细胞质中仅存在稳定的信使RNA时，白蛋白合成增加至总量的63%。这一发现表明白蛋白是在稳定信使RNA上合成的主要蛋白质。
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