Pain V M, Clemens M J, Garlick P J
Biochem J. 1978 Apr 15;172(1):129-35. doi: 10.1042/bj1720129.
In rats fed on a protein-deficient diet, albumin synthesis as a percentage of total liver protein synthesis falls from the normal value of approx. 15% to about 8%. We have extracted total cytoplasmic RNA from individual rat livers and measured the concentration of active albumin mRNA by translation in a reticulocyte lysate system from which the endogenous mRNA had been removed [Pelham & Jackson (1976) Eur. J. Biochem. 67, 247-256]. In this messenger-dependent system it is possible to measure the synthesis of albumin as a proportion of the overall protein synthesis promoted by the addition of the hepatic RNA. The results show that the concentration of translatable albumin mRNA in samples of total cytoplasmic RNA from livers of protein-deficient rats is decreased markedly. These findings suggest that dietary protein supply affects selectively the synthesis and/or functional stability of albumin mRNA in rat liver.
在喂食蛋白质缺乏饮食的大鼠中，白蛋白合成占肝脏总蛋白质合成的百分比从正常的约15%降至约8%。我们从单个大鼠肝脏中提取了总细胞质RNA，并通过在已去除内源性mRNA的网织红细胞裂解物系统中进行翻译来测量活性白蛋白mRNA的浓度[佩勒姆和杰克逊（1976年）《欧洲生物化学杂志》67卷，247 - 256页]。在这个信使依赖性系统中，可以测量添加肝脏RNA后促进的总蛋白质合成中白蛋白的合成比例。结果表明，来自蛋白质缺乏大鼠肝脏的总细胞质RNA样本中可翻译的白蛋白mRNA浓度显著降低。这些发现表明，饮食中的蛋白质供应选择性地影响大鼠肝脏中白蛋白mRNA的合成和/或功能稳定性。
