Institute of Biology, Biotechnology, and Environmental Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland.
Department of Plant Breeding, Physiology, and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, Poland.
Int J Mol Sci. 2020 Jun 16;21(12):4280. doi: 10.3390/ijms21124280.
The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids.
杂种植物的核结构并不是一个研究充分的课题，但它对其遗传稳定性以及在成功表达农艺上所需性状方面具有重要意义。在这项工作中，我们研究了具有从 1 到 4 条添加玉米染色体的燕麦×玉米附加系中渐渗玉米染色质的空间分布。染色体的添加数量通过基因组原位杂交（GISH）来确认。使用玉米染色体特异性简单重复序列（SSR）标记来鉴定添加的染色体。在 3-D 根和叶核上进行 GISH，以评估玉米染色质占据区域的数量、体积和位置。我们揭示了在双二倍体系中存在不同程度的玉米染色体区室（CT）关联，而 CT 分离是在双单体系中最常见的分布模式。在所有分析的系中，玉米 CT 占据的区域优先位于核周缘。对组织的比较表明，与根核相比，叶核中的玉米 CT 更靠近核的中心。这些发现进一步揭示了塑造杂种核结构的过程。
