Extreme Low Cytosolic pH Is a Signal for Cell Survival in Acid Stressed Yeast.

Yeast biomass is recycled in the process of bioethanol production using treatment with dilute sulphuric acid to control the bacterial population. This treatment can lead to loss of cell viability, with consequences on the fermentation yield. Thus, the aim of this study was to define the functional cellular responses to inorganic acid stress. strains with mutation in several signalling pathways, as well as cells expressing pH-sensitive GFP derivative ratiometric pHluorin, were tested for cell survival and cytosolic pH (pH) variation during exposure to low external pH (pH). Mutants in calcium signalling and proton extrusion were transiently sensitive to low pH, while the CWI Δ mutant lost viability. Rescue of this mutant was observed when cells were exposed to extreme low pH or glucose starvation and was dependent on the induced reduction of pH. Therefore, a lowered pH leads to a complete growth arrest, which protects the cells from lethal stress and keeps cells alive. Cytosolic pH is thus a signal that directs the growth stress-tolerance trade-off in yeast. A regulatory model was proposed to explain this mechanism, indicating the impairment of glucan synthesis as the primary cause of low pH sensitivity.