School of Materials and Chemical Engineering, Zhongyuan University of Technology, Zhengzhou, 450007, P.R, China.
Scientific Research Center, Henan University of Chinese Medicine, Zhengzhou, 450046, P.R, China.
Sci Rep. 2020 Jun 19;10(1):10011. doi: 10.1038/s41598-020-67139-7.
Chitosan oligosaccharide functionalized silver nanoparticles with synergistic bacterial activity were constructed as a multivalent inhibitor of bacteria. Placing the chitosan oligosaccharide on silver nanoparticles can dramatically enhance the adsorption to the bacterial membrane via multivalent binding. The multicomponent nanostructures can cooperate synergistically against gram-positive and gram-negative bacteria. The antibacterial activity was increased via orthogonal array design to optimize the synthesis condition. The synergistic bacterial activity was confirmed by fractional inhibitory concentration and zone of inhibition test. Through studies of antimicrobial action mechanism, it was found that the nanocomposites interacted with the bacteria by binding to Mg ions of the bacterial surface. Then, the nanocomposites disrupted bacterial membrane by increasing the permeability of the outer membrane, resulting in leakage of cytoplasm. This strategy of chitosan oligosaccharide modification can increase the antibacterial activity of silver nanoparticles and accelerate wound healing at the same time. The nanomaterial without cytotoxicity has promising applications in bacteria-infected wound healing therapy.
壳寡糖功能化银纳米粒子具有协同抗菌活性，可作为多价细菌抑制剂。壳寡糖置于银纳米粒子上可以通过多价结合显著增强对细菌膜的吸附。多组分纳米结构可以协同对抗革兰氏阳性菌和革兰氏阴性菌。通过正交数组设计来优化合成条件，提高了抗菌活性。协同抗菌活性通过抑菌浓度分数和抑菌圈试验得到证实。通过抗菌作用机制的研究发现，纳米复合材料通过与细菌表面的 Mg 离子结合与细菌相互作用。然后，纳米复合材料通过增加外膜的通透性破坏细菌膜，导致细胞质泄漏。壳寡糖修饰的这种策略可以提高银纳米粒子的抗菌活性，同时加速伤口愈合。这种无细胞毒性的纳米材料在细菌感染的伤口愈合治疗中有很好的应用前景。
