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壳寡糖的抗菌活性，特别关注其抗寄生虫潜力

Antimicrobial Activity of Chitosan Oligosaccharides with Special Attention to Antiparasitic Potential.

作者信息

Silva Nayara Sousa da, Araújo Nathália Kelly, Daniele-Silva Alessandra, Oliveira Johny Wysllas de Freitas, Medeiros Júlia Maria de, Araújo Renata Mendonça, Ferreira Leandro De Santis, Rocha Hugo Alexandre Oliveira, Silva-Junior Arnóbio Antônio, Silva Marcelo Sousa, Fernandes-Pedrosa Matheus de Freitas

机构信息

Postgraduate Program in Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil.

Department of Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil.

出版信息

Mar Drugs. 2021 Feb 12;19(2):110. doi: 10.3390/md19020110.

DOI:10.3390/md19020110
PMID:33673266
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7917997/
Abstract

The global rise of infectious disease outbreaks and the progression of microbial resistance reinforce the importance of researching new biomolecules. Obtained from the hydrolysis of chitosan, chitooligosaccharides (COSs) have demonstrated several biological properties, including antimicrobial, and greater advantage over chitosan due to their higher solubility and lower viscosity. Despite the evidence of the biotechnological potential of COSs, their effects on trypanosomatids are still scarce. The objectives of this study were the enzymatic production, characterization, and in vitro evaluation of the cytotoxic, antibacterial, antifungal, and antiparasitic effects of COSs. NMR and mass spectrometry analyses indicated the presence of a mixture with 81% deacetylated COS and acetylated hexamers. COSs demonstrated no evidence of cytotoxicity upon 2 mg/mL. In addition, COSs showed interesting activity against bacteria and yeasts and a time-dependent parasitic inhibition. Scanning electron microscopy images indicated a parasite aggregation ability of COSs. Thus, the broad biological effect of COSs makes them a promising molecule for the biomedical industry.

摘要

传染病爆发的全球增加以及微生物耐药性的发展强化了研究新生物分子的重要性。壳寡糖（COSs）由壳聚糖水解获得，已证明具有多种生物学特性，包括抗菌特性，并且由于其更高的溶解度和更低的粘度，比壳聚糖具有更大优势。尽管有证据表明COSs具有生物技术潜力，但其对锥虫的影响仍然很少。本研究的目的是对COSs进行酶促生产、表征，并对其细胞毒性、抗菌、抗真菌和抗寄生虫作用进行体外评估。核磁共振（NMR）和质谱分析表明存在一种混合物，其中81%为脱乙酰化COS和乙酰化六聚体。COSs在浓度为2mg/mL时未显示出细胞毒性证据。此外，COSs对细菌和酵母表现出有趣的活性以及时间依赖性的寄生虫抑制作用。扫描电子显微镜图像表明COSs具有寄生虫聚集能力。因此，COSs广泛的生物学效应使其成为生物医学行业中一个有前景的分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/c2b3f843898b/marinedrugs-19-00110-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/27346b05ffa1/marinedrugs-19-00110-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/83919f202f5c/marinedrugs-19-00110-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/f82775f1d409/marinedrugs-19-00110-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/d65935dc4c89/marinedrugs-19-00110-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b15c/7917997/c2b3f843898b/marinedrugs-19-00110-g011.jpg

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