Almeida M A, Politi J R, Ubatuba F B
Farmacologia Médica, Departamento de Medicina Especializada, Universidade de Brasília.
An Acad Bras Cienc. 1988 Sep;60(3):347-53.
This paper presents a method for the screening of natural hypoglycaemic drugs that interfere with the intestinal absorption of glucose. Luminal perfusion of the small intestine (whole length) was carried out on 24 h fasted adult Wistar rats, anaesthetized with sodium pentobarbital. Two rubber Nelaton cannulae were introduced into the organ, the first at the proximal end of the duodenum, just after the pylorus and a second larger one near the ileo-cecal valve. After a preliminary washing with warm physiological saline to remove any alimentary residues and secretions, warm saline containing glucose (plain or with added putative absorption inhibitors), was then introduced into the gut. Ten minutes later the contents was expelled with air and the preparation fully washed with plain warm saline. All perfusates were separately collected up to volume in graduated flasks kept in chipped ice. The glucose concentration was measured in triplicate samples by the specific glucose-oxidase method. The intestinal absorption of the sugar was calculated by difference from the glucose concentration found in the initial solution and in the final perfusate. The method is reliable and highly reproducible.
本文介绍了一种筛选干扰葡萄糖肠道吸收的天然降糖药物的方法。对用戊巴比妥钠麻醉的禁食24小时的成年Wistar大鼠进行小肠（全长）腔内灌注。将两根橡胶内拉通套管插入器官，第一根在十二指肠近端，紧接幽门之后，第二根较大的套管靠近回盲瓣。先用温热的生理盐水初步冲洗以清除任何食物残渣和分泌物，然后将含有葡萄糖（纯葡萄糖或添加了假定吸收抑制剂的葡萄糖）的温热盐水引入肠道。十分钟后，用空气排出内容物，并用纯温热盐水充分冲洗制剂。所有灌流液分别收集到放在碎冰中的刻度烧瓶中直至达到所需体积。通过特定的葡萄糖氧化酶法对一式三份的样品进行葡萄糖浓度测定。通过初始溶液和最终灌流液中葡萄糖浓度的差值计算糖的肠道吸收量。该方法可靠且具有高度可重复性。
