Lv Jinzhi, Yang Wenli, Miao Yanming
Shanxi Normal University, Linfen 041004, PR China.
Shanxi Normal University, Linfen 041004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118599. doi: 10.1016/j.saa.2020.118599. Epub 2020 Jun 11.
Environmental friendly nano biosensor can improve the detection performance of traditional biomolecular sensors and have important application value in practical applications. In this study, a kind of room temperature phosphorescence (RTP) quantum dots (QDs) (GOX RTP QDs) nanobiosensor was prepared by mineralization at room temperature (25 °C), using galactose oxidase (GOX) as template, which improved the catalytic ability of traditional GOx to D-Galactose. The specific enzyme substrate reaction between GOx and D-Galactose and photoinduced electron transfer (Piet) were used to detect the RTP of D-galactose. The linear range of D-galactose detection is 0.02-0.8 mM, and the detection limit of the method is 0.008 mM. This method is based on the RTP property of QDs, which can effectively avoid the interference of background fluorescence of biological samples, and does not need complex sample pretreatment process. Therefore, this method is more suitable for the quantitative detection of D-Galactose in biological samples.
环境友好型纳米生物传感器可以提高传统生物分子传感器的检测性能，在实际应用中具有重要的应用价值。在本研究中，以半乳糖氧化酶（GOX）为模板，通过室温（25℃）矿化制备了一种室温磷光（RTP）量子点（QDs）（GOX RTP QDs）纳米生物传感器，提高了传统GOx对D-半乳糖的催化能力。利用GOx与D-半乳糖之间的特异性酶底物反应和光致电子转移（Piet）来检测D-半乳糖的RTP。D-半乳糖检测的线性范围为0.02-0.8 mM，该方法的检测限为0.008 mM。该方法基于量子点的RTP特性，能有效避免生物样品背景荧光的干扰，且无需复杂的样品预处理过程。因此，该方法更适合生物样品中D-半乳糖的定量检测。
