Fu Xudong, Zhang Chunxia, Zhang Yi
Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115, USA; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA.
Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115, USA; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Boston, MA 02115, USA.
Curr Opin Genet Dev. 2020 Oct;64:13-20. doi: 10.1016/j.gde.2020.05.015. Epub 2020 Jun 18.
After fertilization, mouse embryos go through preimplantation development to give rise to blastocyst. Two key molecular events, zygotic genome activation (ZGA) and the first cell lineage specification, are essential for the process. Recent advances in low-input epigenomics profiling techniques allow the analysis of these events at a molecular level, which revealed a critical role of epigenetic and chromatin reprogramming in ZGA and the first cell lineage specification. Additionally, the establishment of an in vitro embryonic stem cell (ESC) to two-cell embryo-like conversion system have also contributed to the molecular understanding of preimplantation development. In this review, we summarize recent advances in epigenetic regulation of mouse preimplantation development, point out the remaining questions, and propose strategies to tackle these questions.
受精后，小鼠胚胎经历植入前发育，形成囊胚。合子基因组激活（ZGA）和首次细胞谱系特化这两个关键分子事件对该过程至关重要。低投入表观基因组学分析技术的最新进展使得能够在分子水平上分析这些事件，揭示了表观遗传和染色质重编程在ZGA和首次细胞谱系特化中的关键作用。此外，体外胚胎干细胞（ESC）向二细胞胚胎样转化系统的建立也有助于从分子层面理解植入前发育。在本综述中，我们总结了小鼠植入前发育表观遗传调控的最新进展，指出了尚存的问题，并提出了解决这些问题的策略。
