College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Colloids Surf B Biointerfaces. 2020 Oct;194:111168. doi: 10.1016/j.colsurfb.2020.111168. Epub 2020 Jun 2.
It is important to develop effective, biocompatible, easily stored and affordable hemostats for controlling bleeding and preventing infection in prehospital trauma. In this study, we synthesized a series of alginate-based composite microspheres coated by different amounts of berberine (SCC-1B, SCC-5B and SCC-10B), which were further characterized using scanning electron microscopy (SEM), viscometer, particle analyzer and Fourier transform infrared (FT-IR) spectroscopy. The in vitro and vivo results demonstrated that compared to control group (SCC, Composite polysaccharide microspheres without berberine, and CMPHP, Commercial hemostatic agent), SCC-10B with proper content berberine (7%), not only exhibited inherent excellent antibacterial activity, but also enhanced hemostatic effect by increasing adhesion and aggregation of blood cells, which could be considered as synergistic effects. More importantly, through inserting berberine into the cross-linked network, biodegradability and biocompatibility of SCC-10B were also improved. Taken together, SCC-10B could be a candidate for emergency hemostatic and antibacterial treatment in prehospital trauma.
在创伤院前，开发有效、生物相容、易于储存和负担得起的止血剂来控制出血和预防感染非常重要。在这项研究中，我们合成了一系列不同含量小檗碱（SCC-1B、SCC-5B 和 SCC-10B）包被的海藻酸钠基复合微球，并用扫描电子显微镜（SEM）、粘度计、颗粒分析仪和傅里叶变换红外（FT-IR）光谱对其进行了进一步表征。体外和体内结果表明，与对照组（SCC，不含小檗碱的复合多糖微球，和 CMPHP，商业止血剂）相比，含有适量小檗碱（7%）的 SCC-10B 不仅具有内在的优异抗菌活性，而且通过增加血细胞的黏附和聚集来增强止血效果，这可以被认为是协同作用。更重要的是，通过将小檗碱插入交联网络，SCC-10B 的生物降解性和生物相容性也得到了提高。综上所述，SCC-10B 可作为创伤院前紧急止血和抗菌治疗的候选药物。
