Department of Animal Sciences, University of Illinois, Urbana.
J Anim Sci. 2020 Jul 1;98(7). doi: 10.1093/jas/skaa200.
AbstractThree experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA), concentration of metabolizable (ME), and standardized total tract digestibility (STTD) of P in a new source of distillers dried grains with solubles (DDGS; ProCap DDGS) are greater than in conventional de-oiled DDGS. In experiment 1, nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicated 3 × 3 Latin square design with three diets and three periods for a total of nine replicate pigs per diet. Two diets included ProCap DDGS or de-oiled DDGS as the sole source of crude protein (CP) and AA. An N-free diet was used to determine the basal endogenous losses of CP and AA. Ileal digesta were collected on days 5 and 6 of each period after 4 d of adaptation to diets. Results from experiment 1 indicated that ProCap DDGS contained more CP and AA compared with de-oiled DDGS. The SID of all AA in ProCap DDGS was greater (P < 0.001) compared with de-oiled DDGS with the exception that the SID of Pro was not different between the two sources of DDGS. In experiment 2, 24 growing barrows (initial BW: 32.7 ± 3.1 kg) were housed individually in metabolism crates and used in a randomized complete block design and fed a corn-based diet or two diets containing corn and each source of DDGS with eight replicate pigs per diet. Fecal and urine samples were collected for 4 d after 7 d of adaptation. Results from experiment 2 indicated that concentration of ME in ProCap DDGS was greater (P < 0.05) compared with corn or de-oiled DDGS. In experiment 3, 32 growing barrows (initial BW: 20.2 ± 0.9 kg) were placed in metabolism crates and allotted to four diets with eight pigs per diet using a 2 × 2 factorial treatment arrangement. The de-oiled DDGS and ProCap DDGS were both included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Pigs were adapted to the diets for 5 d and fecal samples were collected for 4 d. Results from experiment 3 indicated that inclusion of phytase in the diet containing ProCap DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the de-oiled DDGS diet did not increase STTD of P (interaction, P < 0.001), but the STTD of P was greater (P < 0.05) in de-oiled DDGS compared with ProCap DDGS. In conclusion, ProCap DDGS has greater SID of AA and contains more ME, but has reduced STTD of P compared with conventional de-oiled DDGS.
本研究进行了三项实验,旨在验证一个假设,即新型蒸馏酒糟(ProCap DDGS)中氨基酸(AA)的标准回肠可消化率(SID)、代谢能(ME)浓度和总肠道消化率(STTD)均高于传统脱油 DDGS。实验 1 中,9 头带有回肠 T 型瘘管的阉公猪(初始体重:67.2±6.4kg)被分配到 3×3 拉丁方设计的 3 个处理组和 3 个周期中,每个处理组有 9 个重复猪。两种饲料均以 ProCap DDGS 或脱油 DDGS 作为唯一的粗蛋白(CP)和 AA 来源。无氮饲料用于确定 CP 和 AA 的基础内源性损失。在每个周期的第 4 天适应饲料后,在第 5 和第 6 天收集回肠内容物。实验 1 的结果表明,ProCap DDGS 中的 CP 和 AA 含量高于脱油 DDGS。ProCap DDGS 中所有 AA 的 SID 均显著高于(P<0.001)脱油 DDGS,除 Pro 外,两种 DDGS 来源的 AA SID 没有差异。在实验 2 中,24 头生长育肥猪(初始体重:32.7±3.1kg)被单独饲养在代谢笼中,采用随机完全区组设计,喂食玉米基础日粮或两种含玉米和两种 DDGS 的日粮,每个处理组有 8 个重复猪。适应期 7 天后,收集 4 天的粪便和尿液样本。实验 2 的结果表明,ProCap DDGS 的 ME 浓度显著高于(P<0.05)玉米或脱油 DDGS。在实验 3 中,32 头生长育肥猪(初始体重:20.2±0.9kg)被放置在代谢笼中,采用 2×2 因子处理安排,每组 8 头猪,共 4 个处理组。日粮中同时包含脱油 DDGS 和 ProCap DDGS,以及不含和含 500 单位/kg 日粮的微生物植酸酶。猪适应日粮 5 天,收集 4 天的粪便样本。实验 3 的结果表明,日粮中添加植酸酶增加了 ProCap DDGS 的 STTD,但添加植酸酶对脱油 DDGS 日粮的 STTD 没有影响(互作,P<0.001),但脱油 DDGS 的 STTD 高于 ProCap DDGS。综上所述,ProCap DDGS 的 AA SID 较高,ME 含量较高,但与传统脱油 DDGS 相比,其 STTD 较低。