Tian Wenni, Xiao Nan, Yang Yunyun, Xiao Jie, Zeng Ruiping, Xie Lanhua, Qiu Ziyou, Li Pan, Du Bing
College of Food Science, South China Agricultural University, Guangzhou, Guangdong 510640, China.
Guangdong Engineering and Technology Research Center for Ambient Mass Spectrometry, Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Institute of Analysis (China National Analytical Center Guangzhou), 100 Xianlie Middle Road, Guangzhou 510070, China.
Int J Biol Macromol. 2020 Nov 1;162:116-126. doi: 10.1016/j.ijbiomac.2020.06.150. Epub 2020 Jun 18.
In this study, a novel water-soluble polysaccharide (PVLP-1) was extracted and purified from Sacha inchi (Plukenetia volubilis L.) seeds and the structure, antioxidant and immunomodulatory activity of PVLP-1 were investigated. PVLP-1 (144 kDa) consisted of glucose (69.76%), mannose (14.86%), arabinose (10.53%), galactose (2.42%), ribose (1.23%), rhamnose (0.27%) and xylose (0.93%). PVLP-1 displayed characteristic polysaccharide bands in Fourier transform NMR spectra and infrared. The primary structure of PVLP-1 was a heteropolysaccharide with a backbone of (1 → 6)-linked glucose, sidechains of (1 → 4)-linked mannose, (1 → 4)-linked glucose and (1 → 3, 6)-linked mannose and a residue unit of →1)-linked arabinose as revealed the methylation analysis. PVLP-1 possessed good water-holding capacity (WHC), oil-holding capacity (OHC) and antioxidant capacities. Besides, PVLP-1 induced the proliferation of RAW264.7 cell and enhanced the expression of inflammatory cytokines IL-6, TNF-alpha(TNF-α) and IL-1 beta (IL-1β). The present study indicated that PVLP-1 possessed immune-enhancing bioactivities and could be functional food or adjuvant drug to improve biological immunity of immunodeficiency diseases and hypoimmunity.
在本研究中，从美藤果（Plukenetia volubilis L.）种子中提取并纯化出一种新型水溶性多糖（PVLP-1），并对PVLP-1的结构、抗氧化和免疫调节活性进行了研究。PVLP-1（144 kDa）由葡萄糖（69.76%）、甘露糖（14.86%）、阿拉伯糖（10.53%）、半乳糖（2.42%）、核糖（1.23%）、鼠李糖（0.27%）和木糖（0.93%）组成。PVLP-1在傅里叶变换核磁共振光谱和红外光谱中显示出特征性多糖谱带。甲基化分析表明，PVLP-1的一级结构是一种杂多糖，其主链为（1→6）连接的葡萄糖，侧链为（1→4）连接的甘露糖、（1→4）连接的葡萄糖和（1→3,6）连接的甘露糖，以及一个→1）连接的阿拉伯糖残基单元。PVLP-1具有良好的持水能力（WHC）、持油能力（OHC）和抗氧化能力。此外，PVLP-1诱导RAW264.7细胞增殖，并增强炎症细胞因子IL-6、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的表达。本研究表明，PVLP-1具有免疫增强生物活性，可作为功能性食品或佐剂药物，以改善免疫缺陷疾病和免疫低下的生物免疫力。
