Wei Xinxin, Yao Junxiu, Wang Fangzhou, Wu Dejun, Zhang Rentang
Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, China.
Key Laboratory for Genetics and Breeding in Forest Trees of Shandong Province, Shandong Academy of Forestry Science, Jinan, China.
Front Nutr. 2022 Jul 19;9:947706. doi: 10.3389/fnut.2022.947706. eCollection 2022.
The isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits were studied. Two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4) were isolated from elderberry. EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 all contain arabinose, galactose, glucose, and mannose, with molecular weights of 1.7981 × 10, 7.0523 × 10, 7.7638 × 10, 4.3855 × 10, and 7.3173 × 10 Da, respectively. Structural characterization showed that the backbone of EFP-2 consisted of →4)-Man (1→4)-β-D-Glc (1→ and →4)-β-D-Glc (1→5)-α-L-Ara (1→units, and T-α-L-Ara (1→ and T-β-D-Gal (1→ residues were detected by methylation analysis and NMR analysis. In addition, the MTT assay and zebrafish oxidative damage assay showed that EFP-2 had a protective effect on HO-damaged RAW264.7 cells in a dose-dependent manner, and zebrafish with the addition of EFP-2 would have low levels of ROS which showed significant antioxidant activity. Therefore, the results showed that the elderberry polysaccharides have antioxidant activity and can be used as potential antioxidants in functional foods.
研究了接骨木果实中提取的多糖的分离、纯化及抗氧化活性。从接骨木中分离出两种中性多糖（EFP - 0和EFP - 1）和三种酸性多糖（EFP - 2、EFP - 3和EFP - 4）。EFP - 0、EFP - 1、EFP - 2、EFP - 3和EFP - 4均含有阿拉伯糖、半乳糖、葡萄糖和甘露糖，分子量分别为1.7981×10、7.0523×10、7.7638×10、4.3855×10和7.3173×10 Da。结构表征表明，EFP - 2的主链由→4)-Man(1→4)-β-D-Glc(1→和→4)-β-D-Glc(1→5)-α-L-Ara(1→单元组成，通过甲基化分析和核磁共振分析检测到T-α-L-Ara(1→和T-β-D-Gal(1→残基。此外，MTT法和斑马鱼氧化损伤试验表明，EFP - 2对HO损伤的RAW264.7细胞具有剂量依赖性保护作用，添加EFP - 2的斑马鱼体内活性氧水平较低，显示出显著的抗氧化活性。因此，结果表明接骨木多糖具有抗氧化活性，可作为功能性食品中的潜在抗氧化剂。
