Department of Medicine, College of Medicine, Inha University, Incheon, Republic of Korea.
Indian J Pharmacol. 2020 Mar-Apr;52(2):102-107. doi: 10.4103/ijp.IJP_117_19. Epub 2020 Jun 3.
Glioblastoma is a malignant brain tumor with limited treatment modalities due to its nature. SB365, Pulsatilla saponin D, is known to induce apoptosis and inhibit the growth of many cancer cells.
We elucidated the anticancer effects of SB365 in glioblastoma cells.
We examined the antiproliferative activity of SB365 in human glioblastoma cell lines. Apoptosis was evaluated using the Hoechst assay, TUNEL assay, DAPI nuclear staining, and Western blotting analysis. To test the antimetastatic capacity of SB365, cell migration assay was conducted, and hypoxia-inducible factor-1 alpha (HIF-1α) expression and vascular endothelial growth factor (VEGF) level were determined under hypoxic conditions.
Significance of the results was confirmed by a one-way analysis of variance analysis.
SB365 treatment suppressed the growth of glioblastoma cells and resulted in apoptotic morphological features such as nuclear condensation and fragmentation, enhancing the expression of cleaved poly (ADP-ribose) polymerase and caspase-3. It also significantly delayed cell migration and decreased the HIF-1α expression and VEGF secretion.
Our findings thus demonstrate that SB365 induced apoptosis and delayed the growth and migration of human glioblastoma cells. It is considered that SB365 would be a promising therapeutic option for glioblastoma.
由于其性质，胶质母细胞瘤是一种治疗方法有限的恶性脑肿瘤。白头翁皂苷 D（SB365）已知可诱导细胞凋亡并抑制许多癌细胞的生长。
我们阐明了 SB365 在胶质母细胞瘤细胞中的抗癌作用。
我们检测了 SB365 在人胶质母细胞瘤细胞系中的增殖抑制活性。使用 Hoechst 染色法、TUNEL 检测法、DAPI 核染色和 Western blot 分析评估细胞凋亡。为了测试 SB365 的抗转移能力，进行了细胞迁移测定，并在缺氧条件下测定了缺氧诱导因子-1α（HIF-1α）表达和血管内皮生长因子（VEGF）水平。
通过单向方差分析确认了结果的显著性。
SB365 处理抑制了胶质母细胞瘤细胞的生长，并导致核浓缩和碎裂等凋亡形态特征，增强了裂解聚（ADP-核糖）聚合酶和 caspase-3 的表达。它还显著延迟了细胞迁移，并降低了 HIF-1α 表达和 VEGF 分泌。
因此，我们的研究结果表明，SB365 诱导细胞凋亡并延迟人胶质母细胞瘤细胞的生长和迁移。SB365 被认为是胶质母细胞瘤有前途的治疗选择。
