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PATZ1通过PUMA在胶质母细胞瘤中诱导细胞凋亡。

PATZ1 Induces Apoptosis through PUMA in Glioblastoma.

作者信息

Tao Xiang, Zhang Ge, Liu Junhui, Ji Baowei, Xu Haitao, Chen Zhibiao

机构信息

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060 Hubei Province, China.

出版信息

J Oncol. 2022 Apr 25;2022:4953107. doi: 10.1155/2022/4953107. eCollection 2022.

DOI:10.1155/2022/4953107
PMID:35509848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9061038/
Abstract

AIM

This study was aimed at investigating the mechanism of PATZ1 inducing apoptosis through PUMA in glioblastoma. Overexpressed PATZ1 was transfected to explore its role in inducing apoptosis in glioblastoma cells.

METHODS

The expression of protein was detected by western blotting assay. qRT-PCR assay was used to detect the expression of RNA. Confocal microscopy was used to analyze the correlation between PATZ1 and PUMA. TUNEL assay was used to detect the cell apoptosis. The ability of cell proliferation was detected by MTT assay and EDU assay. The effects of PATZ1 on cell apoptosis and tumor proliferation were observed in vivo by tumor xenograft mouse model.

RESULTS

The results showed that low PATZ1 expression correlates with poor prognosis in glioblastoma patients. Overexpression of PATZ1 inhibits glioma cell proliferation and induces apoptosis by activating intrinsic apoptotic pathways. PATZ1 colocalizes intracellularly with PUMA inducing apoptosis through PUMA in glioblastoma.

CONCLUSION

PATZ1 plays a biological regulatory role in inducing apoptosis in glioblastoma, and this regulatory effect is related to PUMA, and the specific mechanism remains to be further explored.

摘要

目的

本研究旨在探讨PATZ1通过PUMA诱导胶质母细胞瘤细胞凋亡的机制。转染过表达的PATZ1以探究其在诱导胶质母细胞瘤细胞凋亡中的作用。

方法

采用蛋白质免疫印迹法检测蛋白质表达。运用qRT-PCR法检测RNA表达。利用共聚焦显微镜分析PATZ1与PUMA之间的相关性。采用TUNEL法检测细胞凋亡。通过MTT法和EDU法检测细胞增殖能力。利用肿瘤异种移植小鼠模型在体内观察PATZ1对细胞凋亡和肿瘤增殖的影响。

结果

结果显示，胶质母细胞瘤患者中PATZ1低表达与预后不良相关。PATZ1过表达通过激活内源性凋亡途径抑制胶质瘤细胞增殖并诱导凋亡。在胶质母细胞瘤中，PATZ1在细胞内与PUMA共定位，通过PUMA诱导凋亡。

结论

PATZ1在诱导胶质母细胞瘤细胞凋亡中发挥生物学调节作用，且这种调节作用与PUMA有关，具体机制仍有待进一步探究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/8cea8335945e/JO2022-4953107.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/5f62a3388cf8/JO2022-4953107.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/a24d10677d1c/JO2022-4953107.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/b55440359dec/JO2022-4953107.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/c74d98b9a999/JO2022-4953107.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/8ceb37a4b297/JO2022-4953107.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/8cea8335945e/JO2022-4953107.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/5f62a3388cf8/JO2022-4953107.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/a24d10677d1c/JO2022-4953107.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/b55440359dec/JO2022-4953107.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/c74d98b9a999/JO2022-4953107.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/8ceb37a4b297/JO2022-4953107.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5726/9061038/8cea8335945e/JO2022-4953107.006.jpg

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SB365 induces apoptosis and suppresses proliferation of glioblastoma cells.SB365 诱导脑胶质瘤细胞凋亡并抑制其增殖。
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Case report: A unique presentation of a high-grade neuroepithelial tumor with EWSR1::PATZ1 fusion with diagnostic, molecular, and therapeutic insights.病例报告：一例具有EWSR1::PATZ1融合的高级别神经上皮肿瘤的独特表现及诊断、分子和治疗见解。
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