Department of Biomedical Sciences, Inha University, Incheon, Republic of Korea.
Food Chem. 2013 Jan 1;136(1):26-33. doi: 10.1016/j.foodchem.2012.07.096. Epub 2012 Aug 1.
Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.
白头翁已被用作治疗多种疾病的传统药物。本研究旨在确定是否从白头翁根中分离得到的白头翁皂甙 D（SB365）能够抑制结肠癌的进展。我们发现 SB365 强烈抑制结肠癌细胞的生长和增殖，并诱导其凋亡。此外，SB365 通过降低 HIF-1α 和 VEGF 的表达表现出抗血管生成活性。体内研究证实了这些结果，表明 SB365 通过诱导细胞凋亡和抑制血管生成，显著抑制肿瘤生长，其抗癌活性强于 5-FU。当进一步研究其抗癌机制时，SB365 有效地抑制了 AKT/mTOR 通路，无论是在体外还是体内。综上所述，本研究表明 SB365 通过抑制 AKT/mTOR 通路，抑制肿瘤生长和血管生成并诱导细胞凋亡。因此，SB365 是一种很有前途的天然产物，可用于治疗结肠癌。
