Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8656, Japan.
Department of Immunology, Genetics, and Pathology (IGP), Uppsala University, Dag Hammarskjölds väg 20, Uppsala, SE-751 85, Sweden.
Macromol Biosci. 2020 Aug;20(8):e2000114. doi: 10.1002/mabi.202000114. Epub 2020 Jun 22.
Construction of 3D tissues by various types of cells with specific characteristics is an important and fundamental technology in tissue reconstruction medicine and animal-free diagnosis system. To do so, an excellent extracellular matrix (ECM) is needed for encapsulation of cells and maintaining cell activity. Spontaneously forming hydrogel matrix is used by complexation between two water-soluble polymers, 2-methacryloyloxyethyl phosphorylcholine polymer bearing phenylboronic acid groups and poly(vinyl alcohol). Two cytokines for cell proliferation are immobilized in the hydrogel matrix to control the activities of the encapsulated cells. The cytokine-immobilized hydrogel matrix can encapsulate both L929 fibroblasts and normal human dermal fibroblasts under mild condition. The physical properties of the hydrogel matrix can follow the proliferation process of the encapsulated cells. The encapsulated cells secrete ECM in the polymer hydrogel networks upon 3D culturing for 7 days. Consequently, the tissue-mimicking ECM hybrid hydrogels are fabricated successfully.
通过具有特定特征的各种类型的细胞构建 3D 组织是组织重建医学和无动物诊断系统中的一项重要的基础技术。为此，需要一种出色的细胞外基质 (ECM) 来包裹细胞并维持细胞活性。通过带有苯硼酸基团的 2-甲基丙烯酰氧乙基磷酸胆碱聚合物和聚乙烯醇之间的复合物，自发形成水凝胶基质。两种用于细胞增殖的细胞因子被固定在水凝胶基质中，以控制包裹细胞的活性。细胞因子固定的水凝胶基质可以在温和条件下包裹 L929 成纤维细胞和正常人类皮肤成纤维细胞。水凝胶基质的物理性质可以跟随包裹细胞的增殖过程。在 3D 培养 7 天后，包裹的细胞在聚合物水凝胶网络中分泌 ECM。因此，成功制备了模拟组织的 ECM 杂化水凝胶。
