Department of Clinical Investigation, Tripler Army Medical Center, 1 Jarrett White Rd, Building 40, Honolulu, HI, USA.
Department of Clinical Investigation, Tripler Army Medical Center, 1 Jarrett White Rd, Building 40, Honolulu, HI, USA.
Diagn Microbiol Infect Dis. 2020 Aug;97(4):115093. doi: 10.1016/j.diagmicrobio.2020.115093. Epub 2020 May 24.
Development and standardization of simple, timely, and cost-effective antibiotic susceptibility assays are much needed to address the emergence of global resistance. The use of matrix-assisted laser desorption/ionization time of flight mass spectrometry is routine for bacterial identification. This study evaluated 2 assays using the VITEK MS for rapid detection and accurate differentiation of bacterial antibiotic susceptibility. We describe an extraction method and direct-on-target microdroplet growth assay (DOT-MGA). Non-susceptible and susceptible strains of Staphylococcus aureus, Enterococcus species, Escherichia coli, and Klebsiella pneumoniae were tested. The liquid extraction method and DOT-MGA proved to be reliable assays providing consistent differentiation between non-susceptible and susceptible strains. Results from this study support VITEK MS and these assays as rapid and accurate tools to augment traditional susceptibility testing. If implemented clinically, these assays can reduce the cost of patient care and the time to deliver critically needed treatment.
为应对全球耐药性的出现，开发简单、及时且具有成本效益的抗生素药敏检测方法非常必要。基质辅助激光解吸/电离飞行时间质谱法已常规用于细菌鉴定。本研究评估了 2 种使用 VITEK MS 进行快速检测和准确区分细菌抗生素药敏的方法。我们描述了一种提取方法和直接靶标微滴生长检测（DOT-MGA）。对金黄色葡萄球菌、肠球菌属、大肠杆菌和肺炎克雷伯菌的非耐药和敏感菌株进行了测试。液体提取方法和 DOT-MGA 证明是可靠的检测方法，可在非耐药和敏感菌株之间提供一致的区分。本研究结果支持 VITEK MS 和这些检测方法作为快速准确的工具来增强传统药敏检测。如果在临床上实施，这些检测方法可以降低患者治疗成本和获得急需治疗的时间。
