Graduate Program in Dentistry, Pelotas Dental School, UFPel-Federal University of Pelotas, Gonçalves Chaves 457, Pelotas, 96015-560, Rio Grande do Sul, Brazil.
Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, 3570 GreenBrier Boulevard 380 A, 48105, Ann Arbor, MI, USA.
Sci Rep. 2020 Jun 22;10(1):10016. doi: 10.1038/s41598-020-66733-z.
To evaluate the bleaching ability, the effect on enamel surface and cytotoxicity of novel tooth-whitening formulations containing papain, ficin, or bromelain. Forty bovine dental discs (6 cm ×4 cm) were pigmentated and randomly allocated into the following groups (n = 10): Group 1, 20 wt% carbamide peroxide (control); group 2, 1% papain-based whitening; group 3, 1% ficin-based whitening; and group 4, 1% bromelain-based whitening. The whitening gels were prepared and applied on the enamel three times per day once a week, for 4 weeks. Color measurement was obtained by CIEDE2000. Enamel Knoop microhardness and roughness were evaluated. The WST-1 assay was used to evaluate the cell viability of mouse fibroblast cells (L929). Data were statistically analyzed by one-way analysis of variance (ANOVA) and Student Newman Keuls's post hoc test at α = 0.05 significance level. Bromelain, ficin-based, and carbamide peroxide bleaching gels showed a similar color change (p < 0.001). Higher enamel hardness decrease and higher enamel roughness were caused by the carbamide peroxide (p < 0.05). The experimental whitening gels did not affect cell viability. Tooth bleaching gels containing bromelain, papain, or ficin have substantial clinical potential to be used in the development of peroxide-free tooth whitening gels.
为了评估新型含木瓜蛋白酶、ficin 或菠萝蛋白酶的牙齿美白制剂的漂白能力、对牙釉质表面的影响和细胞毒性。将 40 个牛牙圆盘（6cm×4cm）染色，并随机分为以下组（n=10）：第 1 组，20wt%过氧脲（对照）；第 2 组，1%基于木瓜蛋白酶的美白制剂；第 3 组，1%基于 ficin 的美白制剂；第 4 组，1%基于菠萝蛋白酶的美白制剂。每周一次，每天三次将美白凝胶涂抹在牙釉质上，持续 4 周。通过 CIEDE2000 获得颜色测量值。评估牙釉质的 Knoop 显微硬度和粗糙度。使用 WST-1 测定法评估小鼠成纤维细胞（L929）的细胞活力。通过单因素方差分析（ANOVA）和学生纽曼-凯尔斯事后检验对数据进行统计学分析，显著性水平为α=0.05。菠萝蛋白酶、ficin 基和过氧脲美白凝胶显示出相似的颜色变化（p<0.001）。过氧脲引起牙釉质硬度降低和粗糙度增加（p<0.05）。实验性美白凝胶对细胞活力没有影响。含菠萝蛋白酶、木瓜蛋白酶或 ficin 的牙齿美白凝胶具有很大的临床潜力，可用于开发无过氧化物的牙齿美白凝胶。
