转染的浆细胞瘤细胞不会将膜形式的IgM转运至细胞表面。
Transfected plasmacytoma cells do not transport the membrane form of IgM to the cell surface.
作者信息
Hombach J, Sablitzky F, Rajewsky K, Reth M
机构信息
Institute for Genetics, University of Cologne, Federal Republic of Germany.
出版信息
J Exp Med. 1988 Feb 1;167(2):652-7. doi: 10.1084/jem.167.2.652.
Abstract
Expression vectors coding for membrane-bound IgM antibodies were introduced into myeloma and B lymphoma cells. Only the lymphoma but not the myeloma cells were able to express the antibodies on the cell surface, although in both cases, complete antibodies were assembled intracellularly. In myeloma cells, the Ig molecules did not reach the Golgi compartment. Thus, the intracellular transport of membrane-bound antibodies is controlled in the B cell lineages in a developmentally ordered fashion.
摘要
编码膜结合型IgM抗体的表达载体被导入骨髓瘤细胞和B淋巴瘤细胞。只有淋巴瘤细胞而非骨髓瘤细胞能够在细胞表面表达抗体，尽管在这两种情况下，完整的抗体都在细胞内组装完成。在骨髓瘤细胞中，Ig分子无法到达高尔基体区室。因此，膜结合抗体的细胞内运输在B细胞谱系中以发育有序的方式受到调控。
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