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人脂肪细胞条件培养基促进体外成纤维细胞向肌成纤维细胞的转化。

Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts.

机构信息

Roy J. Carver Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USA.

Department of Plastic Surgery, Kindai University, Faculty of Medicine, Higashiosaka, Osaka, Japan.

出版信息

Sci Rep. 2020 Jun 24;10(1):10286. doi: 10.1038/s41598-020-67175-3.

DOI:10.1038/s41598-020-67175-3
PMID:32581231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7314785/
Abstract

Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.

摘要

脂肪细胞和脂肪组织来源的细胞已被研究其在伤口愈合过程中的潜在作用。然而，这些细胞与其他重要细胞类型（如肌成纤维细胞/成纤维细胞）相互作用的细节尚不清楚。使用新型体外 3D 人脂肪细胞/前脂肪细胞球体模型，我们研究了脂肪细胞及其前体（前脂肪细胞）是否分泌影响人真皮成纤维细胞行为的因子。我们发现，脂肪细胞和前脂肪细胞条件培养基均诱导成纤维细胞迁移，但只有脂肪细胞条件培养基诱导成纤维细胞分化为高度收缩、产生胶原的肌成纤维细胞表型。此外，脂肪细胞介导的肌成纤维细胞诱导是通过 TGF-β 独立的机制发生的。我们的发现有助于更好地理解脂肪组织在伤口愈合中的作用，并可能有助于发现和开发与脂肪相关的伤口愈合治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/2c7982afb4b9/41598_2020_67175_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/46fa5533e74b/41598_2020_67175_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/8bc233498960/41598_2020_67175_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/a2c575651062/41598_2020_67175_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/1c55711d087f/41598_2020_67175_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/2c7982afb4b9/41598_2020_67175_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/46fa5533e74b/41598_2020_67175_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/8bc233498960/41598_2020_67175_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/a2c575651062/41598_2020_67175_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/1c55711d087f/41598_2020_67175_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/7314785/2c7982afb4b9/41598_2020_67175_Fig5_HTML.jpg

相似文献

[1]
Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts.

Sci Rep. 2020-6-24

[2]
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[3]
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[4]
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[5]
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J Mol Med (Berl). 2019-3-19

[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
Hyaluronic and Succinic Acid: New Biostimulating Combination to Counteract Dermal and Subcutaneous Aging.

Int J Mol Sci. 2025-8-5

[2]
Fibroblast-Adipocyte Lineage Cell Interactions Result in Differential Production of Extracellular Matrix Proteins.

Cell Mol Bioeng. 2024-10-29

[3]
Mature adipocytes inhibit differentiation of myogenic cells but stimulate proliferation of fibro-adipogenic precursors derived from trout muscle in vitro.

Sci Rep. 2024-7-16

[4]
Development and analysis of scaffold-free adipose spheroids.

Adipocyte. 2024-12

[5]
Native adiponectin plays a role in the adipocyte-mediated conversion of fibroblasts to myofibroblasts.

J R Soc Interface. 2023-5

[6]
Mechanisms underlying pathological scarring by fibroblasts during wound healing.

Int Wound J. 2023-8

[7]
Novel prospects for scarless wound healing: The roles of myofibroblasts and adipocytes.

J Cell Mol Med. 2022-10

[8]
Force-Bioreactor for Assessing Pharmacological Therapies for Mechanobiological Targets.

Front Bioeng Biotechnol. 2022-7-19

[9]
The Prognosis of Arthrofibroses: Prevalence, Clinical Shortcomings, and Future Prospects.

Trends Pharmacol Sci. 2021-5

[10]
Dermal Drivers of Injury-Induced Inflammation: Contribution of Adipocytes and Fibroblasts.

Int J Mol Sci. 2021-2-16

本文引用的文献

[1]
Blebbistatin-Loaded Poly(d,l-lactide--glycolide) Particles For Treating Arthrofibrosis.

ACS Biomater Sci Eng. 2016-7-11

[2]
Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair.

Cell Stem Cell. 2020-6-4

[3]
Fat grafting rescues radiation-induced joint contracture.

Stem Cells. 2020-3

[4]
Sulfasalazine Resolves Joint Stiffness in a Rabbit Model of Arthrofibrosis.

J Orthop Res. 2019-11-11

[5]
Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds.

Nat Commun. 2019-2-8

[6]
Myofibroblast proliferation and heterogeneity are supported by macrophages during skin repair.

Science. 2018-11-23

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Augmentation of Dermal Wound Healing by Adipose Tissue-Derived Stromal Cells (ASC).

Bioengineering (Basel). 2018-10-26

[8]
Effect of adiposity on tissue-specific adiponectin secretion.

PLoS One. 2018-6-20

[9]
Author Correction: Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling.

Sci Rep. 2018-5-1

[10]
MicroRNA-21-5p mediates TGF-β-regulated fibrogenic activation of spinal fibroblasts and the formation of fibrotic scars after spinal cord injury.

Int J Biol Sci. 2018-1-15

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