Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
PLoS One. 2018 Jun 20;13(6):e0198889. doi: 10.1371/journal.pone.0198889. eCollection 2018.
Circulating adiponectin levels are lower in individuals with increased BMI and central adiposity. However, they are paradoxically higher in those with peripheral adiposity. We hypothesized that adiponectin secretion from central and peripheral adipose tissue depots may be associated with adiposity levels and its distribution. A total of 55 subjects (69% women) undergoing elective abdominal surgery (mean age: 53 ± 13 years) were recruited. Health history, anthropometrics, and cardiovascular disease risk factor measurements were obtained. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples were obtained and cultured. Media was collected after 24hr and adiponectin released into the medium was measured using ELISA. We found that mean adiponectin levels from SAT and VAT in all subjects were 17.14±15.27 vs. 15.21±14.28 pg/ml/mg of tissue respectively (p = ns). However, adiponectin secretion from VAT correlated negatively with BMI (r = -0.31, p = 0.01), whereas there was no relationship with SAT (r = 0.08 p = 0.61). Similarly, waist circumference and estimated VAT percentage were both negatively correlated with VAT secretion of adiponectin (r = -0.35, p = 0.01 and r = -0.36, p = 0.02 respectively). These negative correlations were significant only in women on gender-stratified analyses. Adiponectin secretion from VAT decreases with increases in adiposity, while SAT secretion remains unchanged, especially in women. This observation may explain lower circulating adiponectin levels in individuals with central obesity. Further studies are needed to explore the mechanism behind this discrepant adiponectin secretion from SAT and VAT with increases in BMI, particularly among women.
循环脂联素水平在 BMI 增加和中心性肥胖的个体中较低。然而，在那些具有外周性肥胖的个体中，它们却是反常地升高。我们假设来自中央和外周脂肪组织储存的脂联素分泌可能与肥胖水平及其分布有关。总共招募了 55 名（69%为女性）接受择期腹部手术的患者（平均年龄：53 ± 13 岁）。获得了健康史、人体测量学和心血管疾病危险因素测量值。获取了皮下脂肪组织（SAT）和内脏脂肪组织（VAT）样本，并进行培养。收集培养 24 小时后的培养基，并使用 ELISA 测量释放到培养基中的脂联素。我们发现，所有受试者的 SAT 和 VAT 的平均脂联素水平分别为 17.14±15.27pg/ml/mg 组织和 15.21±14.28pg/ml/mg 组织（p = ns）。然而，VAT 的脂联素分泌与 BMI 呈负相关（r = -0.31，p = 0.01），而与 SAT 无相关性（r = 0.08，p = 0.61）。同样，腰围和估计的 VAT 百分比与 VAT 分泌的脂联素均呈负相关（r = -0.35，p = 0.01 和 r = -0.36，p = 0.02）。这些负相关仅在性别分层分析中的女性中具有统计学意义。随着肥胖程度的增加，VAT 的脂联素分泌减少，而 SAT 的分泌保持不变，尤其是在女性中。这种观察结果可以解释为什么中心性肥胖个体的循环脂联素水平较低。需要进一步的研究来探讨 BMI 增加时 SAT 和 VAT 中脂联素分泌不同的机制，尤其是在女性中。
