Wu Qibin, Gao Shiwu, Pan Yong-Bao, Su Yachun, Grisham Michael P, Guo Jinlong, Xu Liping, Que Youxiong
Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Biology and Genetic Breeding, Fuzhou, Fujian, China.
USDA-ARS, Sugarcane Research Unit, Houma, LA, USA.
PeerJ. 2018 Oct 31;6:e5873. doi: 10.7717/peerj.5873. eCollection 2018.
Glyoxalase I belongs to the glyoxalase system that detoxifies methylglyoxal (MG), a cytotoxic by-product produced mainly from triose phosphates. The concentration of MG increases rapidly under stress conditions. In this study, a novel glyoxalase I gene, designated as was identified from sugarcane. had a size of 1,091 bp with one open reading frame (ORF) of 885 bp encoding a protein of 294 amino acids. SoGloI was predicted as a Ni-dependent GLOI protein with two typical glyoxalase domains at positions 28-149 and 159-283, respectively. was cloned into an expression plasmid vector, and the Trx-His-S-tag SoGloI protein produced in was about 51 kDa. The recombinant cells expressing compared to the control grew faster and tolerated higher concentrations of NaCl, CuCl, CdCl, or ZnSO. ubiquitously expressed in various sugarcane tissues. The expression was up-regulated under the treatments of NaCl, CuCl, CdCl, ZnSO and abscisic acid (ABA), or under simulated biotic stress conditions upon exposure to salicylic acid (SA) and methyl jasmonate (MeJA). SoGloI activity steadily increased when sugarcane was subjected to NaCl, CuCl, CdCl, or ZnSO treatments. Sub-cellular observations indicated that the SoGloI protein was located in both cytosol and nucleus. These results suggest that the gene may play an important role in sugarcane's response to various biotic and abiotic stresses.
乙二醛酶I属于乙二醛酶系统，该系统可解毒甲基乙二醛（MG），MG是一种主要由磷酸丙糖产生的细胞毒性副产物。在胁迫条件下，MG的浓度会迅速增加。在本研究中，从甘蔗中鉴定出一个新的乙二醛酶I基因，命名为 。 大小为1091 bp，有一个885 bp的开放阅读框（ORF），编码一个294个氨基酸的蛋白质。SoGloI被预测为一种镍依赖性GLOI蛋白，在第28 - 149位和159 - 283位分别有两个典型的乙二醛酶结构域。 被克隆到表达质粒载体中，在 中产生的Trx - His - S - tag SoGloI蛋白约为51 kDa。与对照相比，表达 的重组 细胞生长更快，并且能耐受更高浓度的NaCl、CuCl、CdCl或ZnSO。 在甘蔗的各种组织中普遍表达。在NaCl、CuCl、CdCl、ZnSO和脱落酸（ABA）处理下，或在暴露于水杨酸（SA）和茉莉酸甲酯（MeJA）后的模拟生物胁迫条件下，其表达上调。当甘蔗受到NaCl、CuCl、CdCl或ZnSO处理时，SoGloI活性稳步增加。亚细胞观察表明，SoGloI蛋白位于细胞质和细胞核中。这些结果表明， 基因可能在甘蔗对各种生物和非生物胁迫的响应中起重要作用。
