van Helden P D, Bester A J
Department of Medical Biochemistry, University of Stellenbosch, Tygerberg, Republic of South Africa.
Biochim Biophys Acta. 1988 Mar 31;949(3):273-8. doi: 10.1016/0167-4781(88)90152-2.
Anti-B-DNA and anti-Z-DNA antibodies were prepared from the serum of systemic lupus erythematosus (SLE) patients by affinity chromatography. The anti-Z-DNA antibodies were shown to exhibit site-specific binding preferences in pBR322 negatively supercoiled (plasmid) DNA, as assayed by restriction-enzyme cleavage. The anti-B-DNA antibodies were found to stimulate in vitro transcription of pBR322, whereas little effect was observed on combination with anti-Z-DNA antibodies. The results support the proposal that the formation of Z-DNA is a down-regulatory mechanism and that the B to Z conformational change may be a flip-flop control for gene expression.
通过亲和层析法从系统性红斑狼疮（SLE）患者的血清中制备抗B - DNA和抗Z - DNA抗体。通过限制性酶切分析表明，抗Z - DNA抗体在pBR322负超螺旋（质粒）DNA中表现出位点特异性结合偏好。发现抗B - DNA抗体可刺激pBR322的体外转录，而与抗Z - DNA抗体结合时观察到的影响很小。这些结果支持了Z - DNA的形成是一种下调机制以及B到Z构象变化可能是基因表达的一种触发控制的提议。
