Price J A, Longbottom J L
Department of Allergy and Clinical Immunology, Cardiothoracic Institute, Brompton Hospital, London, U.K.
Clin Allergy. 1988 Jan;18(1):95-107. doi: 10.1111/j.1365-2222.1988.tb02848.x.
Three types of air-sampling apparatus (the Air-Sentinel, Burkard Personal Sampler and Casella Personal Sampler) were compared for their use in sampling the air in situations where individuals are exposed to laboratory animals, and a cascade impactor was used to determine the sizes of particles carrying the allergens under investigation. An ELISA, using monospecific antisera raised to selected major allergens of the mouse, rat and rabbit, i.e. mouse Ag 1 (mouse urinary prealbumin), rat Ag 4 (rat urinary prealbumin) and rabbit Ag R1, was developed to assay the extracts obtained from the samplers. This ELISA system was able to detect greater than 5 ng/m3 of allergen in sampled air. The rat and mouse allergens were shown to be carried mainly on particles of 6-18 micron, whereas the rabbit allergen was also found on particles of 2 micron and smaller. Allergen levels correlated well with the number of animals present in the room and the degree of activity during sampling. A protective filtered-air hood, when worn during surgical operation procedures, was shown to reduce effectively the level of rat allergen breathed by an individual.
对三种空气采样仪器（空气哨兵、伯卡德个人采样器和卡塞拉个人采样器）在个体接触实验动物的情况下用于空气采样的性能进行了比较，并使用级联冲击器来确定携带所研究过敏原的颗粒大小。开发了一种酶联免疫吸附测定（ELISA）法，使用针对小鼠、大鼠和兔子选定主要过敏原产生的单特异性抗血清，即小鼠抗原1（小鼠尿前白蛋白）、大鼠抗原4（大鼠尿前白蛋白）和兔子抗原R1，来检测从采样器获得的提取物。该ELISA系统能够检测出采样空气中大于5 ng/m³的过敏原。结果表明，大鼠和小鼠过敏原主要附着在6 - 18微米的颗粒上，而兔子过敏原也存在于2微米及更小的颗粒上。过敏原水平与室内动物数量以及采样期间的活动程度密切相关。在外科手术过程中佩戴防护过滤空气头罩，可有效降低个体吸入的大鼠过敏原水平。
