Bender B S, Winchurch R A, Thupari J N, Proust J J, Adler W H, Munster A M
Clinical Immunology Section, National Institute on Aging, Baltimore, MD 21224.
Clin Exp Immunol. 1988 Jan;71(1):120-5.
Natural killer (NK) cells mediate host defense against infections and are regulated by interleukin 2 (IL-2) and other factors. We studied NK cell function in burn patients using a 51Cr release assay with K562 target cells. We found that peripheral blood lymphocytes from burn patients had depressed NK activity (target cell lysis = 22.0 +/- 3.1% vs 39.8 +/- 3.2% in healthy volunteers, P less than 0.001) and also a lower response to IL-2 (28.9 +/- 3.8% vs 53.2 +/- 4.3%, P less than 0.001). Thirteen burn patients were randomly assigned to receive either standard therapy or 5 days of intravenous polymyxin B in addition to standard therapy. After 2 weeks, the patients not receiving polymyxin B had a significant decline in peripheral blood NK activity (P less than 0.01) and response to IL-2 (P less than 0.05), while no decline in NK cell activity was seen in patients who received polymyxin B. Sera from burn patients was found to suppress the NK activity of lymphocytes from healthy adults by 5-75%. After using affinity chromatography to remove endotoxin, the sera from burn patients no longer suppressed NK cell activity. Circulating endotoxin appears to be involved in the suppression of NK activity in burn patients.
自然杀伤（NK）细胞介导宿主抵御感染，并受白细胞介素2（IL-2）和其他因子的调节。我们使用针对K562靶细胞的51Cr释放试验研究了烧伤患者的NK细胞功能。我们发现，烧伤患者的外周血淋巴细胞NK活性降低（靶细胞裂解率为22.0±3.1%，而健康志愿者为39.8±3.2%，P<0.001），并且对IL-2的反应也较低（28.9±3.8%对53.2±4.3%，P<0.001）。13名烧伤患者被随机分配接受标准治疗或除标准治疗外再接受5天静脉注射多粘菌素B。2周后，未接受多粘菌素B的患者外周血NK活性显著下降（P<0.01），对IL-2的反应也下降（P<0.05），而接受多粘菌素B的患者NK细胞活性未见下降。发现烧伤患者的血清可使健康成年人淋巴细胞的NK活性降低5-75%。使用亲和层析去除内毒素后，烧伤患者的血清不再抑制NK细胞活性。循环内毒素似乎参与了烧伤患者NK活性的抑制。
