通过有限稀释分析对人类美洲皮肤利什曼病（巴西利什曼原虫巴西亚种）中利什曼原虫特异性T细胞进行定量分析。
Quantification of Leishmania-specific T cells in human American cutaneous leishmaniasis (Leishmania braziliensis braziliensis) by limiting dilution analysis.
作者信息
Dórea R C, Coutinho S G, Sabroza P C, Hendriks J, Cysne L, Conceição-Silva F
机构信息
Department of Protozoology, WHO Collaborating Centre for Research and Training in Immunology of Parasitic Disease, Rio de Janeiro, Brazil.
出版信息
Clin Exp Immunol. 1988 Jan;71(1):26-31.
Limiting dilution analysis was used to estimate the frequency of Leishmania-specific T cells from the peripheral blood of 18 human cases of American Cutaneous Leishmaniasis (ACL). Sixteen patients had localized cutaneous leishmaniasis (LCL) and two were recovered LCL patients. In 10 patients with active disease and in two with healed lesions the Leishmania-specific T cell frequencies ranged from 1/10(5) to 1/10(3). In six patients no proliferation was detected after 21 days of cell culture. This finding points to very low precursor frequencies in the peripheral blood of these patients. A significant correlation was found between the two groups with low or high Leishmania-specific T cell frequencies and the lymphoproliferative responses to leishmanial antigens. The majority of the blast-like Leishmania-specific T cells showed a helper/inducer (CD4) phenotype.
采用有限稀释分析法，从18例美洲皮肤利什曼病（ACL）患者的外周血中估算利什曼原虫特异性T细胞的频率。16例患者患有局限性皮肤利什曼病（LCL），2例为已康复的LCL患者。在10例活动性疾病患者和2例已愈合病变患者中，利什曼原虫特异性T细胞频率范围为1/10⁵至1/10³。6例患者在细胞培养21天后未检测到增殖。这一发现表明这些患者外周血中的前体细胞频率非常低。在利什曼原虫特异性T细胞频率低或高的两组与对利什曼原虫抗原的淋巴细胞增殖反应之间发现了显著相关性。大多数母细胞样利什曼原虫特异性T细胞表现出辅助/诱导（CD4）表型。
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