Sekkat C, Dornand J, Gerber M
INSERM, Centre Paul Lamarque, Montpellier, France.
Immunology. 1988 Mar;63(3):431-7.
Phytohaemagglutinin (PHA), phorbol myristate acetate (PMA) and PHA + PMA stimulation of T-enriched peripheral blood lymphocytes (PBL) and the Jurkat malignant T-cell line leads to oxidative-product formation, as evaluated by flow cytofluorometric studies, an increase in K+ flux across the membrane, cGMP production and a depolarization of the cell membrane. Irradiation (20 Gy), which enhances IL-2 synthesis by activated T-enriched PBL and Jurkat cells, also increases oxidative product formation, K+ flux, cGMP production, and induces cell membrane depolarization. Conversely, irradiation does not produce a rise in intracellular free Ca2+, as measured in PHA-stimulated Jurkat cells. PMA is also without effect on intracellular free Ca2+, added before or after PHA stimulation. Thus, except for the rise in intracellular free Ca2+, irradiation and stimulation exert similar effects on some of the events observed in IL-2-producing Jurkat cells, but these effects are not additive. Stimulation and irradiation effects are shown to be additive or synergistic only for cGMP production. It is proposed that irradiation may increase IL-2 synthesis by participating in an additional signal related to the oxidative metabolism of arachidonic acid (AA).
通过流式细胞荧光分析研究评估发现，植物血凝素（PHA）、佛波酯（PMA）以及PHA + PMA刺激富含T细胞的外周血淋巴细胞（PBL）和Jurkat恶性T细胞系会导致氧化产物形成、跨膜K + 通量增加、cGMP生成以及细胞膜去极化。辐射（20 Gy）可增强活化的富含T细胞的PBL和Jurkat细胞的IL-2合成，同时也会增加氧化产物形成、K + 通量、cGMP生成，并诱导细胞膜去极化。相反，如在PHA刺激的Jurkat细胞中所测，辐射不会使细胞内游离Ca2 + 升高。在PHA刺激之前或之后添加PMA对细胞内游离Ca2 + 也无影响。因此，除细胞内游离Ca2 + 升高外，辐射和刺激对产生IL-2的Jurkat细胞中观察到的一些事件具有相似作用，但这些作用并非相加性的。刺激和辐射作用仅在cGMP生成方面表现为相加或协同。有人提出，辐射可能通过参与与花生四烯酸（AA）氧化代谢相关的额外信号来增加IL-2合成。
