College of Veterinary Medicine and Agriculture, Addis Ababa University, P.O. Box 34, Bishoftu, Ethiopia.
National Animal Health and Diagnostic Investigation Center, Sebeta, Ethiopia.
Trop Anim Health Prod. 2020 Nov;52(6):3109-3112. doi: 10.1007/s11250-020-02334-2. Epub 2020 Jun 24.
As per the report from the OIE in 2005, infectious laryngotracheitis (ILT) has not been yet reported in Ethiopia. Hence, considering the evident clinical signs on-field associated with the disease, it felt that there is a need to identify the disease and to protect the chicken population. The study was, therefore, aimed at identifying the seroprevalence of ILT virus from the samples collected from chickens in backyard system, so as to notify its prevalence and setup recommendations for further research in the future. Consequently, cross-sectional study was conducted in eleven purposefully selected peasant associations (PA) of Ada'a district from January to May 2019 to determine ILT in backyard chickens. A total number of 426 sera sample of backyard chickens were randomly collected from 11 PA and each sera was exposed to an indirect enzyme-linked immunosorbent assay (iELISA), at the National Animal Health Diagnostic and Investigation Center, Ethiopia. Out of 426 samples, 233 (54.7%) samples were found positive for ILT virus-specific antibody. The highest prevalence was recorded in Wajitu (83.3%), whereas the least was in Giche (40.7%) PA. There was a statistically significant difference (p < 0.05) among seroprevalence and study PA. The result of this study revealed that a high prevalence of ILT virus is circulating among backyard chickens in the selected PA of Ada'a district, which could significantly affect the poultry sector. Thus, further studies on the circulating strains and the epidemiology of the disease should be carried using a molecular diagnostic test.
根据 2005 年世界动物卫生组织（OIE）的报告，埃塞俄比亚尚未报告传染性喉气管炎（ILT）。因此，考虑到现场与该疾病相关的明显临床症状，认为有必要确定该疾病并保护鸡群。因此，本研究旨在从后院系统采集的鸡样本中确定 ILT 病毒的血清流行率，以通知其流行情况，并为今后的进一步研究提出建议。因此，2019 年 1 月至 5 月，在阿达阿区的 11 个有针对性选择的农民协会（PA）中进行了横断面研究，以确定后院鸡中的 ILT。从 11 个 PA 中随机采集了 426 份后院鸡血清样本，并在埃塞俄比亚国家动物健康诊断和调查中心对每份血清样本进行间接酶联免疫吸附试验（iELISA）。在 426 个样本中，有 233 个（54.7%）样本对 ILT 病毒特异性抗体呈阳性。Wajitu（83.3%）的流行率最高，而 Giche（40.7%）的流行率最低。血清流行率和研究 PA 之间存在统计学差异（p < 0.05）。这项研究的结果表明，所选阿达阿区 PA 后院鸡中 ILT 病毒的流行率很高，这可能会对家禽业产生重大影响。因此，应使用分子诊断检测进一步研究疾病的流行株和流行病学。
