Ambrus J L, Jurgensen C H, Witzel N L, Lewis R A, Butler J L, Fauci A S
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
J Immunol. 1988 Apr 1;140(7):2382-8.
Multiple signals are involved in the regulation of Ig production by human B lymphocytes. Leukotrienes, especially LTB4, have been shown to inhibit Ig production by increasing the number and function of suppressor lymphocytes. Production of leukotrienes has been demonstrated by mast cells, basophils, eosinophils, polymorphonuclear leukocytes, monocytes, and macrophages. In this paper we demonstrate that a human T-T hybridoma grown at 5 x 10(5) cells/ml constitutively produces 5 ng/ml of LTC4. Furthermore, we demonstrate that either the supernatant from this hybridoma containing 0.5 to 10 ng/ml LTC4 or purified LTC4 in the range of 0.5 to 5 ng/ml can suppress 50 to 70% of Ig production by unfractionated human mononuclear cells, by normal human cells stimulated with Staphylococcus aureus Cowan I and B cell differentiation factors, and by the EBV-transformed B cell line SKW.6 in the presence of B cell differentiation factors. Thus, LTC4 can have direct effects on B cells and may have a role in normal B cell regulation.
多种信号参与人类B淋巴细胞免疫球蛋白（Ig）产生的调节。白三烯，尤其是LTB4，已被证明可通过增加抑制性淋巴细胞的数量和功能来抑制Ig的产生。肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞、多形核白细胞、单核细胞和巨噬细胞均可产生白三烯。在本文中，我们证明一种以5×10⁵个细胞/毫升生长的人T-T杂交瘤可组成性地产生5纳克/毫升的LTC4。此外，我们还证明，来自该杂交瘤的含有0.5至10纳克/毫升LTC4的上清液，或0.5至5纳克/毫升范围内的纯化LTC4，在存在B细胞分化因子的情况下，可抑制未分级的人单核细胞、受金黄色葡萄球菌Cowan I和B细胞分化因子刺激的正常人细胞以及EB病毒转化的B细胞系SKW.6产生的Ig的50%至70%。因此，LTC4可对B细胞产生直接影响，并可能在正常B细胞调节中发挥作用。
