Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University, Shanghai, 200031, China; Institutes of Biomedical Sciences, Fudan University, Shanghai 200031 China.
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University, Shanghai, 200031, China.
Exp Cell Res. 2020 Sep 15;394(2):112150. doi: 10.1016/j.yexcr.2020.112150. Epub 2020 Jun 22.
Sense of taste is central to evaluate food before digestion. Taste stem cells undergo constant differentiation throughout the life. However, the mechanism underlying the generation of taste receptor cells is still not clear. Here, we cultured taste organoids from either Lgr5+ or Lgr5-cells, and found the preferential generation of Car4+ and Gustducin + taste receptor cells in organoids derived from Lgr5+ cells in circumvallate, foliate or fungiform papillae. Taste organoids derived from Lgr5+ cells in circumvallate papillae of neonatal mice showed stronger capacity to generate taste receptor cells compared to the organoids from Lgr5+ cells of the adult circumvallate papillae. Massive transcriptional differences were found in multiple signaling pathways including taste transduction between organoids derived from circumvallate papillae of adult and neonatal mice. Inhibiting the Notch signaling pathway by LY411575 enhanced taste receptor cell generation in organoids from circumvallate papillae and modulated multiple signaling pathways. Thus, we concluded that receptor cell generation in taste organoids was age-related and regulated via multiple signaling pathways.
味觉是消化前评估食物的核心。味觉干细胞在整个生命过程中不断分化。然而，产生味觉受体细胞的机制仍不清楚。在这里，我们培养了来自 Lgr5+或 Lgr5-细胞的味觉类器官，并发现来自环状、舌状或菌状乳突的 Lgr5+细胞的类器官中优先产生 Car4+和 Gustducin+味觉受体细胞。与来自成年环状乳突的 Lgr5+细胞的类器官相比，来自新生小鼠环状乳突的 Lgr5+细胞的味觉类器官显示出更强的产生味觉受体细胞的能力。在包括味觉转导在内的多个信号通路中，发现了来自成年和新生小鼠环状乳突的类器官之间的大量转录差异。通过 LY411575 抑制 Notch 信号通路可增强来自环状乳突的类器官中的味觉受体细胞生成，并调节多个信号通路。因此，我们得出结论，味觉类器官中的受体细胞生成是与年龄相关的，并通过多种信号通路调节。
