Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Escola de Agronomia, Setor de Melhoramento de Plantas, Universidade Federal de Goiás, Campus II Samambaia, Rodovia Goiânia a Nova Veneza, Goiás, Brazil.
Microb Pathog. 2020 Dec;149:104281. doi: 10.1016/j.micpath.2020.104281. Epub 2020 Jun 23.
Paracoccidioides complex is a genus that comprises pathogenic fungi which are responsible by systemic disease Paracoccidioidomycosis. In host tissues, pathogenic fungi need to acquire nutrients in order to survive, making the uptake of nitrogen essential for their establishment and dissemination. Nitrogen utilization is employed by the alleviation of Nitrogen Catabolite Repression (NCR) which ensures the use of non-preferential or alternative nitrogen sources when preferential sources are not available. NCR is controlled by GATA transcription factors which act through GATA binding sites on promoter regions in NCR-sensitive genes. This process is responsible for encoding proteins involved with the scavenge, uptake and catabolism of a wide variety of non-preferential nitrogen sources. In this work, we predict the existence of AreA GATA transcription factor and feature the zinc finger domain by three-dimensional structure in Paracoccidioides. Furthermore, we demonstrate the putative genes involved with NCR response by means of in silico analysis. The gene expression profile under NCR conditions was evaluated. Demonstrating that P. lutzii supported transcriptional regulation and alleviated NCR in non-preferential nitrogen-dependent medium. The elucidation of NCR in members of Paracoccidioides complex will provide new knowledge about survival, dissemination and virulence for these pathogens with regard to nitrogen-scavenging strategies in the interactions of host-pathogens.
粗球腔菌复合体是一个属，包含了引起系统性疾病球孢子菌病的致病性真菌。在宿主组织中，致病性真菌需要获取营养物质才能生存，因此摄取氮素对于它们的建立和传播是必不可少的。氮素的利用是通过缓解氮分解代谢物阻遏（NCR）来实现的，这确保了当优先氮源不可用时，使用非优先或替代氮源。NCR 由 GATA 转录因子控制，这些转录因子通过 NCR 敏感基因启动子区域上的 GATA 结合位点发挥作用。这个过程负责编码与广泛的非优先氮源的摄取、利用和分解代谢有关的蛋白质。在这项工作中，我们预测了粗球腔菌中存在 AreA GATA 转录因子，并通过三维结构预测了其锌指结构域。此外，我们通过计算机分析证明了与 NCR 反应相关的假定基因的存在。评估了 NCR 条件下的基因表达谱。证明了 P. lutzii 在非优先氮依赖培养基中支持转录调控和缓解 NCR。阐明粗球腔菌复合体成员中的 NCR 将为这些病原体的生存、传播和毒力提供新的知识，这些知识涉及到在宿主-病原体相互作用中氮素获取策略。
