Pulmonology Department-Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group, IMIM-Hospital del Mar, Parc de Salut Mar, Health and Experimental Sciences Department (CEXS), Universitat Pompeu Fabra (UPF), Barcelona Biomedical Research Park (PRBB), 08003 Barcelona, Spain.
Centro de Investigación en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII), 08003 Barcelona, Spain.
Nutrients. 2020 Jun 23;12(6):1870. doi: 10.3390/nu12061870.
We hypothesized that treatment with pharmacological agents known to increase sirtuin-1 activity (resveratrol and curcumin) may enhance muscle regeneration. In limb muscles of mice (C57BL/6J, 10 weeks) exposed to reloading for seven days following a seven-day period of hindlimb immobilization with/without curcumin or resveratrol treatment, progenitor muscle cell numbers (FACS), satellite cell subtypes (histology), early and late muscle regeneration markers, phenotype and morphometry, sirtuin-1 activity and content, and muscle function were assessed. Treatment with either resveratrol or curcumin in immobilized muscles elicited a significant improvement in numbers of progenitor, activated, quiescent, and total counts of muscle satellite cells, compared to non-treated animals. Treatment with either resveratrol or curcumin in reloaded muscles compared to non-treated mice induced a significant improvement in the CSA of both hybrid (curcumin) and fast-twitch fibers (resveratrol), sirtuin-1 activity (curcumin), sirtuin-1 content (resveratrol), and counts of progenitor muscle cells (resveratrol). Treatment with the pharmacological agents resveratrol and curcumin enhanced the numbers of satellite cells (muscle progenitor, quiescent, activated, and total satellite cells) in the unloaded limb muscles but not in the reloaded muscles. These findings have potential clinical implications as treatment with these phenolic compounds would predominantly be indicated during disuse muscle atrophy to enhance the muscle regeneration process.
我们假设，使用已知能增加 SIRT1 活性的药物（白藜芦醇和姜黄素）进行治疗，可能会增强肌肉再生。在经过 7 天的后肢固定（伴或不伴姜黄素或白藜芦醇治疗）后，对重新加载 7 天的小鼠（C57BL/6J，10 周）的肢体肌肉进行研究，评估祖细胞数量（流式细胞术）、卫星细胞亚型（组织学）、早期和晚期肌肉再生标志物、表型和形态计量学、SIRT1 活性和含量以及肌肉功能。与未治疗的动物相比，在固定肌肉中使用白藜芦醇或姜黄素治疗，会显著增加祖细胞、激活的、静止的和总肌肉卫星细胞计数。与未治疗的小鼠相比，在重新加载的肌肉中使用白藜芦醇或姜黄素治疗，会显著增加杂交（姜黄素）和快肌纤维（白藜芦醇）的 CSA、SIRT1 活性（姜黄素）、SIRT1 含量（白藜芦醇）以及祖细胞计数（白藜芦醇）。使用药物白藜芦醇和姜黄素治疗，会增加未负重肢体肌肉中的卫星细胞（肌肉祖细胞、静止的、激活的和总卫星细胞）数量，但不会增加重新加载的肌肉中的卫星细胞数量。这些发现具有潜在的临床意义，因为这些酚类化合物的治疗主要用于失用性肌肉萎缩期间，以增强肌肉再生过程。
