Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group, Pulmonology Department, IMIM-Hospital del Mar, Parc de Salut Mar, 08003 Barcelona, Spain.
Health and Experimental Sciences Department (CEXS), Universitat Pompeu Fabra (UPF), Barcelona Biomedical Research Park (PRBB), 08003 Barcelona, Spain.
Cells. 2021 Sep 15;10(9):2436. doi: 10.3390/cells10092436.
We hypothesized that the phenolic compound resveratrol mitigates muscle protein degradation and loss and improves muscle fiber cross-sectional area (CSA) in gastrocnemius of mice exposed to unloading (7dI). In gastrocnemius of mice (female C57BL/6J, 10 weeks) exposed to a seven-day period of hindlimb immobilization with/without resveratrol treatment, markers of muscle proteolysis (tyrosine release, systemic troponin-I), atrophy signaling pathways, and muscle phenotypic features and function were analyzed. In gastrocnemius of unloaded mice treated with resveratrol, body and muscle weight and function were attenuated, whereas muscle proteolysis (tyrosine release), proteolytic and apoptotic markers, atrophy signaling pathways, and myofiber CSA significantly improved. Resveratrol treatment of mice exposed to a seven-day period of unloading prevented body and muscle weight and limb strength loss, while an improvement in muscle proteolysis, proteolytic markers, atrophy signaling pathways, apoptosis, and muscle fiber CSA was observed in the gastrocnemius muscle. These findings may have potential therapeutic implications in the management of disuse muscle atrophy in clinical settings.
我们假设酚类化合物白藜芦醇可以减轻肌肉蛋白质的降解和损失，并改善接受去负荷（7dI）处理的小鼠腓肠肌的肌纤维横截面积（CSA）。在接受为期 7 天的后肢固定和/或白藜芦醇处理的雌性 C57BL/6J 小鼠（10 周龄）的腓肠肌中，分析了肌肉蛋白水解（酪氨酸释放，全身肌钙蛋白 I）、萎缩信号通路以及肌肉表型特征和功能的标志物。在接受白藜芦醇处理的去负荷小鼠的腓肠肌中，体重和肌肉重量及功能减弱，而肌肉蛋白水解（酪氨酸释放）、蛋白水解和凋亡标志物、萎缩信号通路和肌纤维 CSA 显著改善。在接受为期 7 天去负荷处理的小鼠中，白藜芦醇处理可预防体重和肌肉重量及肢体力量的丧失，而腓肠肌中的肌肉蛋白水解、蛋白水解标志物、萎缩信号通路、凋亡和肌纤维 CSA 得到改善。这些发现可能对临床环境中废用性肌肉萎缩的管理具有潜在的治疗意义。
