Department of Animal and Pre-veterinary Studies, University of Findlay, Findlay OH, 45840, USA.
Department of Animal and Pre-veterinary Studies, University of Findlay, Findlay OH, 45840, USA.
Reprod Biol. 2020 Sep;20(3):315-320. doi: 10.1016/j.repbio.2020.06.002. Epub 2020 Jun 22.
Elevated levels of reactive oxygen species can cause oxidative stress, which could lead to membrane damage, decreased fertility, and spermatozoan morphological deformities. Antioxidants can be supplemented to reduce the impacts of oxidative stress. The objective of this study was to determine the effects of supplementing quercetin (0.25, 0.50, 0.75 mM) during the thawing and incubation of frozen-thawed boar semen on spermatozoan characteristics, IVF kinetics (n = 400) and subsequent embryonic development (n = 1340). Spermatozoa were evaluated for motility, viability, and membrane lipid peroxidation levels at 0, 2, 4, 6, 8, and 10 h after thawing. Embryos were evaluated for IVF kinetics 12 h after IVF (penetration, polyspermy, male pronucleus formation, IVF efficiency) and cleavage and blastocyst formation at 48 h and 144 h after IVF, respectively. Spermatozoa supplemented with 0.25 mM quercetin had significantly higher (P < 0.05) motility (51.67±8.50 %) and percent of viable cells (61.21 ± 2.44 %) compared to all other treatments at 10 h after thawing, in addition to having significantly (P < 0.05) lower levels of hydroperoxide (3.38 ± 0.88 μM/10cells). There were no differences in penetration rates and male pronucleus formation between treatment groups. Supplementation of quercetin significantly decreased (P < 0.05) polyspermy and significantly increased (P < 0.05) the percentage of embryos reaching blastocyst stage of development by 144 h after IVF compared to no supplementation. Results indicated that supplementing frozen-thawed boar semen with 0.25 mM quercetin improves sperm characteristics up to 10 h after thawing and decreases polyspermy while improving early embryonic development in pigs.
高水平的活性氧会导致氧化应激，从而导致膜损伤、生育力下降和精子形态畸形。抗氧化剂可以被补充以减少氧化应激的影响。本研究的目的是确定在冷冻解冻猪精液解冻和孵育过程中补充槲皮素（0.25、0.50、0.75mM）对精子特征、体外受精动力学（n=400）和随后胚胎发育（n=1340）的影响。在解冻后 0、2、4、6、8 和 10 小时评估精子的运动性、活力和膜脂质过氧化水平。在 IVF 后 12 小时评估胚胎的 IVF 动力学（穿透、多精受精、雄性原核形成、IVF 效率）和分别在 IVF 后 48 小时和 144 小时评估胚胎的卵裂和囊胚形成。与其他所有处理相比，补充 0.25mM 槲皮素的精子在解冻后 10 小时具有更高的运动性（51.67±8.50%）和活细胞比例（61.21±2.44%），此外，羟自由基水平也显著降低（3.38±0.88μM/10 个细胞）（P<0.05）。处理组之间的穿透率和雄性原核形成率没有差异。与不补充相比，补充槲皮素可显著降低（P<0.05）多精受精，并显著增加（P<0.05）IVF 后 144 小时达到囊胚发育阶段的胚胎比例。结果表明，在冷冻解冻猪精液中补充 0.25mM 槲皮素可提高解冻后 10 小时内精子的特性，并减少多精受精，同时提高猪的早期胚胎发育。
