儿童髓母细胞瘤的长非编码 RNA 谱分析。

Long non-coding RNA profiling of pediatric Medulloblastoma.

机构信息

Child Health Research Institute, University of Nebraska Medical Center, Omaha, NE, 69198, USA.

Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, 69198, USA.

出版信息

BMC Med Genomics. 2020 Jun 26;13(1):87. doi: 10.1186/s12920-020-00744-7.

BACKGROUND

Medulloblastoma (MB) is one of the most common malignant cancers in children. MB is primarily classified into four subgroups based on molecular and clinical characteristics as (1) WNT (2) Sonic-hedgehog (SHH) (3) Group 3 (4) Group 4. Molecular characteristics used for MB classification are based on genomic and mRNAs profiles. MB subgroups share genomic and mRNA profiles and require multiple molecular markers for differentiation from each other. Long non-coding RNAs (lncRNAs) are more than 200 nucleotide long RNAs and primarily involve in gene regulation at epigenetic and post-transcriptional levels. LncRNAs have been recognized as diagnostic and prognostic markers in several cancers. However, the lncRNA expression profile of MB is unknown.

METHODS

We used the publicly available gene expression datasets for the profiling of lncRNA expression across MB subgroups. Functional analysis of differentially expressed lncRNAs was accomplished by Ingenuity pathway analysis (IPA).

RESULTS

In the current study, we have identified and validated the lncRNA expression profile across pediatric MB subgroups and associated molecular pathways. We have also identified the prognostic significance of lncRNAs and unique lncRNAs associated with each MB subgroup.

CONCLUSIONS

Identified lncRNAs can be used as single biomarkers for molecular identification of MB subgroups that warrant further investigation and functional validation.

背景

髓母细胞瘤（MB）是儿童中最常见的恶性癌症之一。MB 主要根据分子和临床特征分为四个亚组：（1）WNT （2）Sonic-hedgehog（SHH）（3）Group 3 （4）Group 4。用于 MB 分类的分子特征基于基因组和 mRNAs 图谱。MB 亚组共享基因组和 mRNA 图谱，需要多个分子标记物才能彼此区分。长非编码 RNA（lncRNA）是长度超过 200 个核苷酸的 RNA，主要参与表观遗传和转录后水平的基因调控。lncRNA 已被认为是几种癌症的诊断和预后标志物。然而，MB 的 lncRNA 表达谱尚不清楚。