Department of Biochemistry, Faculty of Science, Helwan University, Cairo, Egypt.
Department of Environmental & Occupational Medicine, National Research Centre, Cairo, Egypt.
Asian Pac J Cancer Prev. 2020 Jun 1;21(6):1689-1695. doi: 10.31557/APJCP.2020.21.6.1689.
Colorectal cancer (CRC) is the most common type of gastrointestinal tract cancers. This investigation aim was to assess the expression of miR-576-3p and miR-613 in CRC patients in addition to NDRG2 and YKL40 serum levels determination to decide their diagnostic and prognostic significance.
Sixty early diagnosed CRC patients prior to any treatment in addition to twelve healthy subjects were enrolled in this study. Blood samples were taken from subjects and allowed for clotting and centrifugation, then the collected sera were stored at -80ºC till it were used for detection of our molecular biomarkers. The mature miRNAs expressions (miR-576-3p and miR-613) were detected in serum by qRT-PCR, while NDRG2 and YKL40 serum levels were determined by ELISA. In addition, the correlation of the measured parameters with the clinicopathological data of the patients was investigated.
The study results showed that both miRNA-576-3p and miRNA-613 were down-regulated in CRC patients with fold change 0.33, 0.36; respectively. A significant positive correlation was observed between miR-576-3p and miR-613 (r = 0.75, p < 0.001). NDRG2 serum levels were decreased in patients compared to the control group but the decrease wasn't statistically significant. On the other hand, it was observed that YKL40 serum level was significantly increased in CRC patients compared to control (p-value < 0.001). Furthermore, YKL40 showed a very high diagnostic value (AUC = 0.97, specificity = 91.7%, sensitivity = 96%, p-value = 0.0001).
The observations of this investigation concluded that, the expressions of miR-576-3p and miR-613 in addition to YKL40 serum levels determinations may help in the diagnosis of CRC.
结直肠癌（CRC）是最常见的胃肠道癌症类型。本研究旨在评估 miR-576-3p 和 miR-613 在 CRC 患者中的表达，以及 NDRG2 和 YKL40 血清水平的测定，以确定其诊断和预后意义。
本研究纳入了 60 例早期诊断的 CRC 患者（未经任何治疗）和 12 例健康对照者。采集受试者血液样本，待血液凝固和离心后，收集血清并储存在-80°C，直至用于检测我们的分子生物标志物。通过 qRT-PCR 检测血清中成熟 miRNA（miR-576-3p 和 miR-613）的表达，通过 ELISA 检测 NDRG2 和 YKL40 血清水平。此外，还研究了所测参数与患者临床病理数据的相关性。
研究结果显示，miR-576-3p 和 miR-613 在 CRC 患者中均下调， fold change 分别为 0.33 和 0.36。miR-576-3p 和 miR-613 之间存在显著正相关（r = 0.75，p < 0.001）。与对照组相比，NDRG2 血清水平在患者中降低，但差异无统计学意义。另一方面，观察到 YKL40 血清水平在 CRC 患者中显著升高（p 值<0.001）。此外，YKL40 具有非常高的诊断价值（AUC = 0.97，特异性 = 91.7%，灵敏度 = 96%，p 值 = 0.0001）。
本研究观察到，miR-576-3p 和 miR-613 的表达以及 YKL40 血清水平的测定可能有助于 CRC 的诊断。
